Porcine circovirus type 2 induces the activation of nuclear factor kappa B by I{kappa}B{alpha} degradation
Abstract
The transcription factor NF-{kappa}B is commonly activated upon virus infection and a key player in the induction and regulation of the host immune response. The present study demonstrated for the first time that porcine circovirus type 2 (PCV2), which is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome, can activate NF-{kappa}B in PCV2-infected PK15 cells. In PCV2-infected cells, NF-{kappa}B was activated concomitantly with viral replication, which was characterized by increased DNA binding activity, translocation of NF-{kappa}B p65 from the cytoplasm to the nucleus, as well as degradation and phosphorylation of I{kappa}B{alpha} protein. We further demonstrated PCV2-induced activation of NF-{kappa}B and colocalization of p65 nuclear translocation with virus replication in cultured cells. Treatment of cells with CAPE, a selective inhibitor of NF-{kappa}B activation, reduced virus protein expression and progeny production followed by decreasing PCV2-induced apoptotic caspase activity, indicating the involvement of this transcription factor in induction of cell death. Taken together, these data suggest that NF-{kappa}B activation is important for PCV2 replication and contributes to virus-mediated changes in host cells. The results presented here provide a basis for understanding molecular mechanism of PCV2 infection.
- Authors:
-
- Institute of Animal Husbandry and Veterinary Medicine, Beijing Municipal Academy of Agriculture and Forestry Sciences, No.9 Shuguang Garden Central Road, Haidian District, Beijing 100097 (China)
- Animal Health Biotechnology Group, Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604 (Singapore)
- Publication Date:
- OSTI Identifier:
- 21141030
- Resource Type:
- Journal Article
- Journal Name:
- Virology
- Additional Journal Information:
- Journal Volume: 378; Journal Issue: 1; Other Information: DOI: 10.1016/j.virol.2008.05.013; PII: S0042-6822(08)00340-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; APOPTOSIS; CYTOPLASM; DNA; PHOSPHORYLATION; SWINE; TRANSCRIPTION FACTORS; TRANSLOCATION; VIRUSES
Citation Formats
Li, Wei, Kwang, Jimmy, Jin, Wang, Lei, Shi, Bing, Yang, Yongqing, Li, and Liu Jue. Porcine circovirus type 2 induces the activation of nuclear factor kappa B by I{kappa}B{alpha} degradation. United States: N. p., 2008.
Web. doi:10.1016/j.virol.2008.05.013.
Li, Wei, Kwang, Jimmy, Jin, Wang, Lei, Shi, Bing, Yang, Yongqing, Li, & Liu Jue. Porcine circovirus type 2 induces the activation of nuclear factor kappa B by I{kappa}B{alpha} degradation. United States. https://doi.org/10.1016/j.virol.2008.05.013
Li, Wei, Kwang, Jimmy, Jin, Wang, Lei, Shi, Bing, Yang, Yongqing, Li, and Liu Jue. 2008.
"Porcine circovirus type 2 induces the activation of nuclear factor kappa B by I{kappa}B{alpha} degradation". United States. https://doi.org/10.1016/j.virol.2008.05.013.
@article{osti_21141030,
title = {Porcine circovirus type 2 induces the activation of nuclear factor kappa B by I{kappa}B{alpha} degradation},
author = {Li, Wei and Kwang, Jimmy and Jin, Wang and Lei, Shi and Bing, Yang and Yongqing, Li and Liu Jue},
abstractNote = {The transcription factor NF-{kappa}B is commonly activated upon virus infection and a key player in the induction and regulation of the host immune response. The present study demonstrated for the first time that porcine circovirus type 2 (PCV2), which is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome, can activate NF-{kappa}B in PCV2-infected PK15 cells. In PCV2-infected cells, NF-{kappa}B was activated concomitantly with viral replication, which was characterized by increased DNA binding activity, translocation of NF-{kappa}B p65 from the cytoplasm to the nucleus, as well as degradation and phosphorylation of I{kappa}B{alpha} protein. We further demonstrated PCV2-induced activation of NF-{kappa}B and colocalization of p65 nuclear translocation with virus replication in cultured cells. Treatment of cells with CAPE, a selective inhibitor of NF-{kappa}B activation, reduced virus protein expression and progeny production followed by decreasing PCV2-induced apoptotic caspase activity, indicating the involvement of this transcription factor in induction of cell death. Taken together, these data suggest that NF-{kappa}B activation is important for PCV2 replication and contributes to virus-mediated changes in host cells. The results presented here provide a basis for understanding molecular mechanism of PCV2 infection.},
doi = {10.1016/j.virol.2008.05.013},
url = {https://www.osti.gov/biblio/21141030},
journal = {Virology},
issn = {0042-6822},
number = 1,
volume = 378,
place = {United States},
year = {Fri Aug 15 00:00:00 EDT 2008},
month = {Fri Aug 15 00:00:00 EDT 2008}
}