Calcium dependent and independent cytokine synthesis by air pollution particle-exposed human bronchial epithelial cells

Journal Article · Sat Dec 01 00:00:00 EST 2007 · Toxicology and Applied Pharmacology

DOI:https://doi.org/10.1016/j.taap.2007.07.006· OSTI ID:21077860

Sakamoto, Noriho; Hayashi, Shizu; Gosselink, John ^[1]; Ishii, Hiroshi ^[2]; Ishimatsu, Yuji ^[1]; Mukae, Hiroshi ^[2]; Hogg, James C ^[1]; Eeden, Stephan F. van , ^[1]

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1. James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, University of British Columbia, St. Paul's Hospital, Vancouver, BC (Canada)
2. Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki (Japan)

Exposure to ambient air pollution particles with a diameter of < 10 {mu}m (PM{sub 10}) has been associated with increased cardiopulmonary morbidity and mortality. We have shown that human bronchial epithelial cells (HBECs) exposed to PM{sub 10} produce pro-inflammatory mediators that contribute to a local and systemic inflammatory response. Changes in intracellular calcium concentrations ([Ca{sup 2+}]{sub i}) have been demonstrated to regulate several functions of the airway epithelium including the production of pro-inflammatory mediators. The aim of the present study was to determine the nature and mechanism of calcium responses induced by PM{sub 10} in HBECs and its relationship to cytokine synthesis. Methods: Primary HBECs were exposed to urban air pollution particles (EHC-93) and [Ca{sup 2+}]{sub i} responses were measured using the fluoroprobe (Fura-2). Cytokine levels were measured at mRNA and protein levels using real-time PCR and ELISA. Results: PM{sub 10} increased [Ca{sup 2+}]{sub i} in a dose-dependent manner. This calcium response was reduced by blocking the influx of calcium into cells (i.e. calcium-free medium, NiCl{sub 2}, LaCl{sub 3}). PM{sub 10} also decreased the activity of calcium pumps. PM{sub 10} increased the production of IL-1{beta}, IL-8, GM-CSF and LIF. Preincubation with intracellular calcium chelator (BAPTA-AM) attenuated IL-1{beta} and IL-8 production, but not GM-CSF and LIF production. Conclusion: We conclude that exposure to PM{sub 10} induces an increase in cytosolic calcium and cytokine production in bronchial epithelial cells. Our results also suggest that PM{sub 10} induces the production of pro-inflammatory mediators via either intracellular calcium-dependent (IL-1{beta}, IL-8) or -independent (GM-CSF, LIF) pathways.

• OSTI ID: 21077860
• Journal Information: Toxicology and Applied Pharmacology, Vol. 225, Issue 2; Other Information: DOI: 10.1016/j.taap.2007.07.006; PII: S0041-008X(07)00319-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AIR POLLUTION
CALCIUM
CALCIUM IONS
CESIUM FLUORIDES
DISEASE INCIDENCE
ENZYME IMMUNOASSAY
EPITHELIUM
INFLAMMATION
LANTHANUM CHLORIDES
LITHIUM FLUORIDES
MORTALITY
NICKEL CHLORIDES
POLYMERASE CHAIN REACTION
PROTEINS