The design and performance of an In-field ESI-FTICR instrument

Wu, Qinyuan; Udseth, H R; Anderson, G A

Title: The design and performance of an In-field ESI-FTICR instrument

Full Record
Other Related Research

Abstract

The coupling of electrospray ionization (ESI) with Fourier Transform Ion Cyclotron Resonance mass spectrometry (FTICR-MS) has been shown to be a promising tool for biological research. Most of the current schemes of combining ESI with FTICR involve forming ions outside the magnet and injecting into the magnetic field through either radio frequency ion guides or electrostatic lenses. Substantial ion losses occur during ion transportation for such external source ESI-FTICR, offsetting the high sensitivity in detection. Alternatively, ESI can be performed inside the magnet bore to significantly increase ion current in the cell. However, such an in-field ESI scheme requires critical differential pumping and ion optics design to maintain ultra-high vacuum in the cell region in order to achieve resolution similar to that obtained from external source scheme. In our in-field ESI-FTICR instrument, a home-made ESI probe is inserted into a superconductor magnet (max. 4.7 T, but presently at 1 T) so that electrospray generated ions are confined by the magnetic field and are injected into the ICR cell located 20 cm from the ESI source.

Authors:

Wu, Qinyuan; Udseth, H R; Anderson, G A ^[1]

Pacific Northwest Lab., Richland, WA (United States); and others

Publication Date:: Sun Dec 31 00:00:00 EST 1995

OSTI Identifier:: 210720

Report Number(s):: CONF-9505261-
TRN: 96:001157-0159

Resource Type:: Conference

Resource Relation:: Conference: 43. American Society of Mass Spectrometry (ASMS) conference on mass spectrometry and allied topics, Atlanta, GA (United States), 21-26 May 1995; Other Information: PBD: 1995; Related Information: Is Part Of Proceedings of the 43rd ASMS conference on mass spectrometry and allied topics; PB: 1411 p.

Country of Publication:: United States

Language:: English

Subject:: 44 INSTRUMENTATION, INCLUDING NUCLEAR AND PARTICLE DETECTORS; MASS SPECTROMETERS; PERFORMANCE; MASS SPECTROSCOPY; DESIGN; ION CYCLOTRON RESONANCE SPECTROSCOPY

Citation Formats


                    Wu, Qinyuan, Udseth, H R, and Anderson, G A. The design and performance of an In-field ESI-FTICR instrument.  United States: N. p., 1995. 
        Web.

Copy to clipboard


                    Wu, Qinyuan, Udseth, H R, & Anderson, G A. The design and performance of an In-field ESI-FTICR instrument.  United States.

Copy to clipboard


                    Wu, Qinyuan, Udseth, H R, and Anderson, G A. 1995.  
        "The design and performance of an In-field ESI-FTICR instrument".  United States.

Copy to clipboard


                    
@article{osti_210720,

  title        = {The design and performance of an In-field ESI-FTICR instrument},

  author       = {Wu, Qinyuan and Udseth, H R and Anderson, G A},

  abstractNote = {The coupling of electrospray ionization (ESI) with Fourier Transform Ion Cyclotron Resonance mass spectrometry (FTICR-MS) has been shown to be a promising tool for biological research. Most of the current schemes of combining ESI with FTICR involve forming ions outside the magnet and injecting into the magnetic field through either radio frequency ion guides or electrostatic lenses. Substantial ion losses occur during ion transportation for such external source ESI-FTICR, offsetting the high sensitivity in detection. Alternatively, ESI can be performed inside the magnet bore to significantly increase ion current in the cell. However, such an in-field ESI scheme requires critical differential pumping and ion optics design to maintain ultra-high vacuum in the cell region in order to achieve resolution similar to that obtained from external source scheme. In our in-field ESI-FTICR instrument, a home-made ESI probe is inserted into a superconductor magnet (max. 4.7 T, but presently at 1 T) so that electrospray generated ions are confined by the magnetic field and are injected into the ICR cell located 20 cm from the ESI source.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/210720},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 00:00:00 EST 1995},

  month        = {Sun Dec 31 00:00:00 EST 1995}

}

Copy to clipboard

Conference:

Other availability

Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

High-Throughput Proteomics Using High Efficiency Multiple-Capillary Liquid Chromatography With On-Line High-Performance ESI FTICR Mass Spectrometry

Journal Article Shen, Yufeng; Tolic, Nikola; Zhao, Rui; ... - Analytical Chemistry

We report on the design and application of a high-efficiency multiple-capillary liquid chromatography (LC) system for high-throughput proteome analysis. The multiple-capillary LC system was operated at the pressure of 10,000 psi using commercial LC pumps to deliver the mobile phase and newly developed passive feedback valves to switch the mobile phase flow and introduce samples. The multiple-capillary LC system was composed of several serially connected dual-capillary column devices. The dual-capillary column approach was designed to eliminate the time delay for regeneration (or equilibrium) of the capillary column after its use under the mobile phase gradient condition (i.e. one capillary columnmore »« less
A new dual cell trap for selective ion accumulation and separation in ESI-FTICR mass spectrometry

Conference Chen, R; Bruce, J; Cheng, Xueheng

The selective accumulation from externally generated low ion currents in an FTICR mass spectrometer can be effectively accomplished. The selective ion accumulation process can facilitate many types of studies, and is particularly effective in conjunction with continuous injection from an electrospray source. However, this technique also requires relative high background gas pressure ({ge} 10{sup -5} Torr), which can limit the optimal performance for subsequent FTICR measurements at pressure of {approximately}10{sup -8} Torr and below. To resolve these conflicting demands, the authors have designed a new dual cell-type of ion trap. The first cell is sealed in a box which ismore » slightly larger than the cell itself.« less
CITP ESI-FTICR for the study of X-irradiation damaged tetranucleotides

Conference Hofstadler, S; Zhao, Zhongxi; Smith, R

A unique advantage of capillary isotachophoresis (CITP) is the ability to pre-concentrate low-level analytes and subsequently perform high resolution separations starting with relatively large (and dilute) sample volumes. The inherent isoelectric focusing and purification common to CITP make it well suited for trace analysis. The coupling of this technique to a high performance mass spectrometric detection scheme provides an unprecedented opportunity to obtain molecular weight and structural information from analytes which are present in a concentration limited regime. The authors have recently combined CITP with electrospray ionization (ESI) Fourier transform ion cyclotron resonance (FTICR) mass spectrometry in order to analyzemore »« less
Proofreading activity of Pfu thermostable DNA polymerase on a 6-O-Methylguanine Containing Template Monitored by ESI-FTICR Mass Spectrometry.

Journal Article Wunschel, David; Masselon, Christophe; Feng, Bingbing; ... - Chembiochem

DNA damage can take the form of chemical lesions that interfere with DNA polymerization and therefore, the replication of DNA within a cell. In this report we examine the effect of a particular type of base modification, a 6-O-methyl group on a guanine base. Previous reports using different DNA polymerases have identified an induced base substitution. However, this process has not been studied using polymerase chain reaction (PCR) enzyme. Electrospray ionization (ESI) mass spectrometry (MS) was used to examine the effect of this type of base on the PCR. Using Fourier transform ion cyclotron resonance (FTICR) MS, two types ofmore »« less
https://doi.org/10.1002/cbic.200400059
Retrieval of DNA using soft-landing after mass analysis by ESI-FTICR for enzymatic manipulation

Journal Article Feng, B; Wunschel, D; Masselon, C; ... - Journal of the American Chemical Society

The DNA modifications that lead to mutations are of direct interest for, e.g. the understanding of DNA damage recognition and repair. The characterization of the modified oligonucleotides and enzymatic responses to specific DNA modifications are of primary concern, but also present major analytical challenges. Among the available techniques, mass spectrometry has become an increasingly important tool for the study of oligonucleotides, their mutations, and interactions. Conventionally, mass spectrometry provides mass and structural information (e.g. from dissociation experiments and the use of tandem mass spectrometry). However, the small quantities of material analyzed and the destructive nature of conventional mass spectrometric detectionmore »« less
https://doi.org/10.1021/ja9923888

Similar Records