Effects of high glucose on mesenchymal stem cell proliferation and differentiation
- Orthopedic Center for Musculoskeletal Research, Orthopedic Department, University of Wuerzburg, Brettreichstrasse 11, D-97074 Wuerzburg (Germany)
- Division of Endocrinology and Diabetes, University of Freiburg (Germany)
- Department of Endocrinology, University Hospital of Odense, Odense (Denmark)
High glucose (HG) concentrations impair cellular functions and induce apoptosis. Exposition of mesenchymal stem cells (MSC) to HG was reported to reduce colony forming activity and induce premature senescence. We characterized the effects of HG on human MSC in vitro using telomerase-immortalized MSC (hMSC-TERT) and primary MSC (hMSC). HG (25 mM) enhanced hMSC-TERT proliferation in long-term studies in contrast to hMSC where proliferation was unchanged. Thioredoxin-interacting protein, which is involved in apoptosis regulation, was stimulated by glucose in hMSC-TERT. However, apoptosis was not influenced by HG in both cell types. MSC treatment with HG favored osteogenic differentiation. MSC are resistant to HG toxicity, depending on the stemness of MSC. Proliferation and osteogenic differentiation are stimulated by HG. Effects of HG on the transient amplifying compartment of MSC may differ from those in mature cells. Further research is needed to unravel the molecular mechanisms of HG resistance of MSC.
- OSTI ID:
- 21032967
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 363, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2007.08.161; PII: S0006-291X(07)01889-X; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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