Cloning and characterization of human RTVP-1b, a novel splice variant of RTVP-1 in glioma cells
- Hermelin Brain Tumor Center, Department of Neurosurgery, Henry Ford Health System, Detroit, MI 48202 (United States)
- Mina and Everard Goodman Faculty of Life-Sciences, Bar-Ilan University, Ramat-Gan 52900 (Israel)
Here, we report the cloning and characterization of RTVP-1b, a novel splice variant of human RTVP-1, which was isolated from the U87 glioma cell line. Sequence analysis revealed that RTVP-1b contains an additional 71 base exon between exons 2 and 3 that is missing in RTVP-1, leading to a frame-shift and a different putative protein. The deduced protein was 237 amino acids in length, sharing the N-terminal 141 amino acids with RTVP-1. RT-PCR analysis demonstrated that RTVP-1b was expressed in a wide range of tissues and that its expression was different from that of RTVP-1. In contrast, RTVP-1 and RTVP-1b showed similar patterns of expression in astrocytic tumors; highly expressed in glioblastomas as compared to normal brains, low-grade astrocytomas and anaplastic oligodendrogliomas. Overexpression of RTVP-1b increased glioma cell proliferation but did not affect cell migration. Our results suggest that RTVP-1b represents a potential prognostic marker and therapeutic target in gliomas.
- OSTI ID:
- 21032941
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 362, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2007.08.138; PII: S0006-291X(07)01751-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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