Cholesterol biosensor based on rf sputtered zinc oxide nanoporous thin film

Journal Article · Mon Aug 06 00:00:00 EDT 2007 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.2768302· OSTI ID:21016095

Singh, S P; Arya, Sunil K; Pandey, Pratibha; Malhotra, B D; Saha, Shibu; Sreenivas, K; Gupta, Vinay ^[1]

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1. BECPRG, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012 (India)

Cholesterol oxidase (ChOx) has been immobilized onto zinc oxide (ZnO) nanoporous thin films grown on gold surface. A preferred c-axis oriented ZnO thin film with porous surface morphology has been fabricated by rf sputtering under high pressure. Optical studies and cyclic voltammetric measurements show that the ChOx/ZnO/Au bioelectrode is sensitive to the detection of cholesterol in 25-400 mg/dl range. A relatively low value of enzyme's kinetic parameter (Michaelis-Menten constant) {approx}2.1 mM indicates enhanced enzyme affinity of ChOx to cholesterol. The observed results show promising application of nanoporous ZnO thin film for biosensing application without any functionalization.

• OSTI ID: 21016095
• Journal Information: Applied Physics Letters, Vol. 91, Issue 6; Other Information: DOI: 10.1063/1.2768302; (c) 2007 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
BIOPHYSICS
CHEMICAL ANALYSIS
CHOLESTEROL
DEPOSITION
GOLD
MORPHOLOGY
OXIDASES
POROUS MATERIALS
SEMICONDUCTOR MATERIALS
SPUTTERING
THIN FILMS
ZINC OXIDES