Cloning and characterization of BmK86, a novel K{sup +}-channel blocker from scorpion venom
Abstract
Scorpion venom represents a tremendous hitherto unexplored resource for understanding ion channels. BmK86 is a novel K{sup +}-channel toxin gene isolated from a cDNA library of Mesobuthus martensii Karsch, which encodes a signal peptide of 22 amino acid residues and a mature toxin of 35 residues with three disulfide bridges. The genomic sequence of BmK86 consists of two exons disrupted by an intron of 72 bp. Comparison with the other scorpion toxins BmK86 shows low sequence similarity. The GST-BmK86 fusion protein was successfully expressed in Escherichia coli. The fusion protein was cleaved by enterokinase and the recombinant BmK86 was purified by HPLC. Using whole-cell patch-clamp recording, the recombinant BmK86 was found to inhibit the potassium current of mKv1.3 channel expressed in COS7 cells. These results indicated that BmK86 belongs to a representative member of a novel subfamily of {alpha}-KTxs. The systematic number assigned to BmK86 is {alpha}-KTx26.1.
- Authors:
-
- State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072 (China)
- Publication Date:
- OSTI Identifier:
- 20991520
- Resource Type:
- Journal Article
- Journal Name:
- Biochemical and Biophysical Research Communications
- Additional Journal Information:
- Journal Volume: 360; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.06.108; PII: S0006-291X(07)01335-6; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; AMINO ACIDS; DISULFIDES; ESCHERICHIA COLI; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDES; POTASSIUM; POTASSIUM IONS; SCORPIONS; TOXINS; VENOMS
Citation Formats
Mao, Xin, Cao, Zhijian, Yin, Shijin, Ma, Yibao, Wu, Yingliang, and Li, Wenxin. Cloning and characterization of BmK86, a novel K{sup +}-channel blocker from scorpion venom. United States: N. p., 2007.
Web. doi:10.1016/j.bbrc.2007.06.108.
Mao, Xin, Cao, Zhijian, Yin, Shijin, Ma, Yibao, Wu, Yingliang, & Li, Wenxin. Cloning and characterization of BmK86, a novel K{sup +}-channel blocker from scorpion venom. United States. https://doi.org/10.1016/j.bbrc.2007.06.108
Mao, Xin, Cao, Zhijian, Yin, Shijin, Ma, Yibao, Wu, Yingliang, and Li, Wenxin. 2007.
"Cloning and characterization of BmK86, a novel K{sup +}-channel blocker from scorpion venom". United States. https://doi.org/10.1016/j.bbrc.2007.06.108.
@article{osti_20991520,
title = {Cloning and characterization of BmK86, a novel K{sup +}-channel blocker from scorpion venom},
author = {Mao, Xin and Cao, Zhijian and Yin, Shijin and Ma, Yibao and Wu, Yingliang and Li, Wenxin},
abstractNote = {Scorpion venom represents a tremendous hitherto unexplored resource for understanding ion channels. BmK86 is a novel K{sup +}-channel toxin gene isolated from a cDNA library of Mesobuthus martensii Karsch, which encodes a signal peptide of 22 amino acid residues and a mature toxin of 35 residues with three disulfide bridges. The genomic sequence of BmK86 consists of two exons disrupted by an intron of 72 bp. Comparison with the other scorpion toxins BmK86 shows low sequence similarity. The GST-BmK86 fusion protein was successfully expressed in Escherichia coli. The fusion protein was cleaved by enterokinase and the recombinant BmK86 was purified by HPLC. Using whole-cell patch-clamp recording, the recombinant BmK86 was found to inhibit the potassium current of mKv1.3 channel expressed in COS7 cells. These results indicated that BmK86 belongs to a representative member of a novel subfamily of {alpha}-KTxs. The systematic number assigned to BmK86 is {alpha}-KTx26.1.},
doi = {10.1016/j.bbrc.2007.06.108},
url = {https://www.osti.gov/biblio/20991520},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 360,
place = {United States},
year = {Fri Sep 07 00:00:00 EDT 2007},
month = {Fri Sep 07 00:00:00 EDT 2007}
}