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Title: PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide

Abstract

Native LDL would be a mitogenic and chemotactic stimulus of VSMC proliferation and differentiation in the atherosclerotic lesion where endothelial disruption occurred. In previous studies, our group investigated the molecular mechanisms by which LDL induces IL-8 production and by which PPAR{alpha} activation abolishes LDL effects in human aortic SMCs (hAoSMCs). Herein is the first report of PPAR{gamma} activation by troglitazone (TG) exerting its inhibitory effects on LDL-induced cell proliferation via generation not of H{sub 2}O{sub 2}, but of O2?-, and the subsequent activation of Erk1/2 in hAoSMCs. Moreover, in this study TG abolished the LDL-accelerated G{sub 1}-S progression to control levels via down-regulation of active cyclinD1/CDK4 and cyclinE/CDK2 complexes and up-regulation of p21{sup Cip1} expression. TG exerted its anti-proliferative effects through the up-regulation of basal superoxide dismutase (SOD) expression. This data suggests that the regulation of O2?- is located at the crossroads between LDL signaling and cell proliferation.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [1]
  1. Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon (Korea, Republic of)
  2. Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong, Daejeon (Korea, Republic of)
  3. Department of Pharmacy, Chungnam National University, Yuseong, Daejeon (Korea, Republic of)
Publication Date:
OSTI Identifier:
20991485
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 359; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2007.06.006; PII: S0006-291X(07)01231-4; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ARTERIOSCLEROSIS; CELL PROLIFERATION; GENE REGULATION; HYDROGEN PEROXIDE; LIPOPROTEINS; MUSCLES; SUPEROXIDE DISMUTASE

Citation Formats

Heo, Kyung-Sun, Department of Pharmacy, Chungnam National University, Yuseong, Daejeon, Kim, Dong-Uk, Ryoo, Sungwoo, Nam, Miyoung, Baek, Seung Tae, Kim, Lila, Park, Song-Kyu, Myung, Chang-Seon, and Hoe, Kwang-Lae. PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.06.006.
Heo, Kyung-Sun, Department of Pharmacy, Chungnam National University, Yuseong, Daejeon, Kim, Dong-Uk, Ryoo, Sungwoo, Nam, Miyoung, Baek, Seung Tae, Kim, Lila, Park, Song-Kyu, Myung, Chang-Seon, & Hoe, Kwang-Lae. PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide. United States. https://doi.org/10.1016/j.bbrc.2007.06.006
Heo, Kyung-Sun, Department of Pharmacy, Chungnam National University, Yuseong, Daejeon, Kim, Dong-Uk, Ryoo, Sungwoo, Nam, Miyoung, Baek, Seung Tae, Kim, Lila, Park, Song-Kyu, Myung, Chang-Seon, and Hoe, Kwang-Lae. 2007. "PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide". United States. https://doi.org/10.1016/j.bbrc.2007.06.006.
@article{osti_20991485,
title = {PPAR{gamma} activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide},
author = {Heo, Kyung-Sun and Department of Pharmacy, Chungnam National University, Yuseong, Daejeon and Kim, Dong-Uk and Ryoo, Sungwoo and Nam, Miyoung and Baek, Seung Tae and Kim, Lila and Park, Song-Kyu and Myung, Chang-Seon and Hoe, Kwang-Lae},
abstractNote = {Native LDL would be a mitogenic and chemotactic stimulus of VSMC proliferation and differentiation in the atherosclerotic lesion where endothelial disruption occurred. In previous studies, our group investigated the molecular mechanisms by which LDL induces IL-8 production and by which PPAR{alpha} activation abolishes LDL effects in human aortic SMCs (hAoSMCs). Herein is the first report of PPAR{gamma} activation by troglitazone (TG) exerting its inhibitory effects on LDL-induced cell proliferation via generation not of H{sub 2}O{sub 2}, but of O2?-, and the subsequent activation of Erk1/2 in hAoSMCs. Moreover, in this study TG abolished the LDL-accelerated G{sub 1}-S progression to control levels via down-regulation of active cyclinD1/CDK4 and cyclinE/CDK2 complexes and up-regulation of p21{sup Cip1} expression. TG exerted its anti-proliferative effects through the up-regulation of basal superoxide dismutase (SOD) expression. This data suggests that the regulation of O2?- is located at the crossroads between LDL signaling and cell proliferation.},
doi = {10.1016/j.bbrc.2007.06.006},
url = {https://www.osti.gov/biblio/20991485}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 359,
place = {United States},
year = {Fri Aug 10 00:00:00 EDT 2007},
month = {Fri Aug 10 00:00:00 EDT 2007}
}