Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer

Binato, Renata; Pizzatti, Luciana; Abdelhay, Eliana

doi:10.1016/j.bbrc.2007.04.190

Title: Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer

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Abstract

A comparative analysis between sequences of Msx1 promoter gene from human, mouse, and fugu allowed us to identify sequences highly conserved among these animals. One of the regions of great homology is localized between the positions -4622 and -4572, including the region described as distal enhancer. In this region putative transcription factors binding sites for Nkx2.5, CTF-CBP, Bicoid, Brn2, and Oct were found. To evaluate the functionality of these sites we performed EMSA analysis using two different regions from the distal enhancer and nuclear protein extracts from embryos. The results showed that in the presence of a Bicoid consensus binding site a DNA-protein complex can be formed. The identification of the proteins involved in this complex by mass spectrometry and Western blotting identified OTX2, a Bicoid-like protein. This protein was shown to be present in nuclear extracts of the embryonic stages analyzed by Western blot. Altogether these results suggest that OTX2 is a putative candidate to activate mice Msx1 gene from distal enhancer.

Authors:

Binato, Renata ^[1]; Pizzatti, Luciana ^[1]; Abdelhay, Eliana ^[1]

National Institute of Cancer, Bone Marrow Transplantation Unit, RJ (Brazil)

Publication Date:: Fri Jun 29 00:00:00 EDT 2007

OSTI Identifier:: 20991422

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 358; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.04.190; PII: S0006-291X(07)00934-5; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; DNA; EMBRYOS; GENES; MASS SPECTROSCOPY; MICE; PROMOTERS; TRANSCRIPTION FACTORS

Citation Formats


                    Binato, Renata, Pizzatti, Luciana, and Abdelhay, Eliana. Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer.  United States: N. p., 2007. 
        Web.  doi:10.1016/j.bbrc.2007.04.190.

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                    Binato, Renata, Pizzatti, Luciana, & Abdelhay, Eliana. Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer.  United States.  https://doi.org/10.1016/j.bbrc.2007.04.190

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                    Binato, Renata, Pizzatti, Luciana, and Abdelhay, Eliana. 2007.  
        "Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer".  United States.  https://doi.org/10.1016/j.bbrc.2007.04.190.

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@article{osti_20991422,

  title        = {Otx2 is a putative candidate to activate mice Msx1 gene from distal enhancer},

  author       = {Binato, Renata and Pizzatti, Luciana and Abdelhay, Eliana},

  abstractNote = {A comparative analysis between sequences of Msx1 promoter gene from human, mouse, and fugu allowed us to identify sequences highly conserved among these animals. One of the regions of great homology is localized between the positions -4622 and -4572, including the region described as distal enhancer. In this region putative transcription factors binding sites for Nkx2.5, CTF-CBP, Bicoid, Brn2, and Oct were found. To evaluate the functionality of these sites we performed EMSA analysis using two different regions from the distal enhancer and nuclear protein extracts from embryos. The results showed that in the presence of a Bicoid consensus binding site a DNA-protein complex can be formed. The identification of the proteins involved in this complex by mass spectrometry and Western blotting identified OTX2, a Bicoid-like protein. This protein was shown to be present in nuclear extracts of the embryonic stages analyzed by Western blot. Altogether these results suggest that OTX2 is a putative candidate to activate mice Msx1 gene from distal enhancer.},

  doi          = {10.1016/j.bbrc.2007.04.190},

  url          = {https://www.osti.gov/biblio/20991422},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 2,

  volume       = 358,

  place        = {United States},

  year         = {Fri Jun 29 00:00:00 EDT 2007},

  month        = {Fri Jun 29 00:00:00 EDT 2007}

}

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