The rare crystallographic structure of d(CGCGCG){sub 2}: The natural spermidine molecule bound to the minor groove of left-handed Z-DNA d(CGCGCG){sub 2} at 10 {sup o}C
- Osaka University of Pharmaceutical Sciences, 4-20-1, Nasahara, Takatsuki, Osaka 569-1094 (Japan)
- The Institute of Scientific and Industrial Research, Osaka University, 8-1, Mihogaoka, Ibaragi, Osaka 567-0047 (Japan)
Several crystal structure analyses of complexes of synthetic polyamine compounds, including N {sup 1}-(2-(2-aminoethylamino))ethyl)ethane-1,2-diamine PA(222) and N {sup 1}-(2-(2-(2-aminoethylamino)ethylamino)ethyl)ethane-1,2-diamine PA(2222), and left-handed Z-DNA d(CGCGCG){sub 2} have been reported. However, until now, there have been no examples of naturally occurring polyamines bound to the minor groove of the left-handed Z-DNA of d(CGCGCG){sub 2} molecule. We have found that spermidine, a natural polyamine, is connected to the minor groove of left-handed Z-DNA of d(CGCGCG){sub 2} molecule in a crystalline complex grown at 10 {sup o}C. The electron density of the DNA molecule was clear enough to determine that the spermidine was connected in the minor groove of two symmetry related molecules of left-handed Z-DNA d(CGCGCG){sub 2}. This is the first example that a spermidine molecule can form a bridge conformation between two symmetry related molecules of left-handed Z-DNA d(CGCGCG){sub 2} in the minor groove.
- OSTI ID:
- 20991394
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 358, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2007.04.026; PII: S0006-291X(07)00713-9; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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