Mtf-1 lymphoma-susceptibility locus affects retention of large thymocytes with high ROS levels in mice after {gamma}-irradiation

Journal Article · Fri Mar 02 00:00:00 EST 2007 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.12.192· OSTI ID:20979818

Maruyama, Masaki ^[1]; Yamamoto, Takashi ^[1]; Kohara, Yuki ^[1]; Katsuragi, Yoshinori ^[1]; Mishima, Yukio ^[1]; Aoyagi, Yutaka ^[2]; Kominami, Ryo ^[1]; niigata-u ac jp, E-mail: rykomina@med

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1. Department of Molecular Genetics, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Niigata 951-8510 (Japan)
2. Third Department of Internal Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata 951-8510 (Japan)

Mouse strains exhibit different susceptibilities to {gamma}-ray-induced thymic lymphomas. Our previous study identified Mtf-1 (metal responsive transcription factor-1) as a candidate susceptibility gene, which is involved in the radiation-induced signaling pathway that regulates the cellular reactive oxygen species (ROS). To reveal the mechanism for the increased susceptibility conferred by Mtf-1 locus, we examined early effects of {gamma}-ray on ROS levels in vivo and its difference between Mtf-1 susceptible and resistant congenic mice. Here, we show the detection of clonally growing thymocytes at 4 weeks after irradiation, indicating the start of clonal expansion at a very early stage. We also show that large thymocytes with higher ROS levels and a proliferation capacity were more numerous in the Mtf-1 susceptible mice than the resistant mice when examined at 7 days after irradiation, although such tendency was not found in mice lacking one allele of Bcl11b tumor suppressor gene. This high retention of the large thymocytes, at a high risk for ROS-induced mutation, is a compensatory proliferation and regeneration response to depletion of the thymocytes after irradiation and the response is likely to augment the development of prelymphoma cells leading to thymic lymphomas.

• OSTI ID: 20979818
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 354, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.192; PII: S0006-291X(06)02862-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
CELL PROLIFERATION
GAMMA RADIATION
IRRADIATION
LYMPHOMAS
MICE
THYMOCYTES
TRANSCRIPTION FACTORS