Structural investigations on native collagen type I fibrils using AFM

Strasser, Stefan; Zink, Albert; Janko, Marek; Heckl, Wolfgang M; Thalhammer, Stefan

doi:10.1016/j.bbrc.2006.12.114

Title: Structural investigations on native collagen type I fibrils using AFM

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Abstract

This study was carried out to determine the elastic properties of single collagen type I fibrils with the use of atomic force microscopy (AFM). Native collagen fibrils were formed by self-assembly in vitro characterized with the AFM. To confirm the inner assembly of the collagen fibrils, the AFM was used as a microdissection tool. Native collagen type I fibrils were dissected and the inner core uncovered. To determine the elastic properties of collagen fibrils the tip of the AFM was used as a nanoindentor by recording force-displacement curves. Measurements were done on the outer shell and in the core of the fibril. The structural investigations revealed the banding of the shell also in the core of native collagen fibrils. Nanoindentation experiments showed the same Young's modulus on the shell as well as in the core of the investigated native collagen fibrils. In addition, the measurements indicate a higher adhesion in the core of the collagen fibrils compared to the shell.

Authors:

Strasser, Stefan ^[1]; Zink, Albert ^[1]; Janko, Marek ^[1]; Heckl, Wolfgang M ^[1]; Thalhammer, Stefan ^[2]

Department of Geo- and Environmental Sciences, Ludwig-Maximilians-Universitaet, 80333 Munich (Germany)
GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg (Germany)

Publication Date:: Fri Mar 02 00:00:00 EST 2007

OSTI Identifier:: 20979812

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 354; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.12.114; PII: S0006-291X(06)02759-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ATOMIC FORCE MICROSCOPY; COLLAGEN; ELASTICITY; IN VITRO; MORPHOLOGY; SPECTROSCOPY; YOUNG MODULUS

Citation Formats


                    Strasser, Stefan, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Zink, Albert, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Janko, Marek, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Heckl, Wolfgang M, Deutsches Museum, Museumsinsel 1, 80538 Munich, and Thalhammer, Stefan. Structural investigations on native collagen type I fibrils using AFM.  United States: N. p., 2007. 
        Web.  doi:10.1016/j.bbrc.2006.12.114.

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                    Strasser, Stefan, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Zink, Albert, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Janko, Marek, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Heckl, Wolfgang M, Deutsches Museum, Museumsinsel 1, 80538 Munich, & Thalhammer, Stefan. Structural investigations on native collagen type I fibrils using AFM.  United States.  https://doi.org/10.1016/j.bbrc.2006.12.114

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                    Strasser, Stefan, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Zink, Albert, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Janko, Marek, GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg, Heckl, Wolfgang M, Deutsches Museum, Museumsinsel 1, 80538 Munich, and Thalhammer, Stefan. 2007.  
        "Structural investigations on native collagen type I fibrils using AFM".  United States.  https://doi.org/10.1016/j.bbrc.2006.12.114.

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@article{osti_20979812,

  title        = {Structural investigations on native collagen type I fibrils using AFM},

  author       = {Strasser, Stefan and GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg and Zink, Albert and GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg and Janko, Marek and GSF-National Research Center for Environment and Health, Institute of Radiation Protection, AG Nanoanalytics, 85764 Neuherberg and Heckl, Wolfgang M and Deutsches Museum, Museumsinsel 1, 80538 Munich and Thalhammer, Stefan},

  abstractNote = {This study was carried out to determine the elastic properties of single collagen type I fibrils with the use of atomic force microscopy (AFM). Native collagen fibrils were formed by self-assembly in vitro characterized with the AFM. To confirm the inner assembly of the collagen fibrils, the AFM was used as a microdissection tool. Native collagen type I fibrils were dissected and the inner core uncovered. To determine the elastic properties of collagen fibrils the tip of the AFM was used as a nanoindentor by recording force-displacement curves. Measurements were done on the outer shell and in the core of the fibril. The structural investigations revealed the banding of the shell also in the core of native collagen fibrils. Nanoindentation experiments showed the same Young's modulus on the shell as well as in the core of the investigated native collagen fibrils. In addition, the measurements indicate a higher adhesion in the core of the collagen fibrils compared to the shell.},

  doi          = {10.1016/j.bbrc.2006.12.114},

  url          = {https://www.osti.gov/biblio/20979812},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 1,

  volume       = 354,

  place        = {United States},

  year         = {Fri Mar 02 00:00:00 EST 2007},

  month        = {Fri Mar 02 00:00:00 EST 2007}

}

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