Early depletion of proliferating B cells of germinal center in rapidly progressive simian immunodeficiency virus infection

Journal Article · Thu May 10 00:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.12.006· OSTI ID:20977022

Zhiqiang, Zhang ^[1]; Casimiro, Danilo R ^[1]; Schleif, William A ^[1]; Chen, Minchun ^[1]; Citron, Michael ^[1]; Davies, Mary-Ellen ^[1]; Burns, Janine ^[1]; Liang, Xiaoping ^[1]; Fu, Tong-Ming ^[1]; Handt, Larry ^[1]; Emini, Emilio A ^[1]; Shiver, John W ^[1]

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1. Department of Vaccines and Biologics Research, Merck Research Laboratories, West Point, PA 19486 (United States)

Lack of virus specific antibody response is commonly observed in both HIV-1-infected humans and SIV-infected monkeys with rapid disease progression. However, the mechanisms underlying this important observation still remain unclear. In a titration study of a SIVmac239 viral stock, three out of six animals with viral inoculation rapidly progressed to AIDS within 5 months. Unexpectedly, there was no obvious depletion of CD4{sup +} T cells in both peripheral and lymph node (LN) compartments in these animals. Instead, progressive depletion of proliferating B cells and disruption of the follicular dendritic cell (FDC) network in germinal centers (GC) was evident in the samples collected at as early as 20 days after viral challenge. This coincided with undetectable, or weak and transient, virus-specific antibody responses over the course of infection. In situ hybridization of SIV RNA in the LN samples revealed a high frequency of SIV productively infected cells and large amounts of accumulated viral RNA in the GCs in these animals. Early severe depletion of GC proliferating B cells and disruption of the FDC network may thus result in an inability to mount a virus-specific antibody response in rapid progressors, which has been shown to contribute to accelerated disease progression of SIV infection.

• OSTI ID: 20977022
• Journal Information: Virology, Vol. 361, Issue 2; Other Information: DOI: 10.1016/j.virol.2006.12.006; PII: S0042-6822(06)00891-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AIDS
AIDS VIRUS
ANTIBODIES
DENDRITES
IN-SITU HYBRIDIZATION
INOCULATION
LYMPH NODES
MONKEYS
RNA
TITRATION
TRANSIENTS