Meliae cortex extract exhibits anti-allergic activity through the inhibition of Syk kinase in mast cells
- College of Medicine, Konkuk University, Chungju 380-701 (Korea, Republic of)
- School of Bioscience and Biotechnology, Kangwon National University, Gangwon-do 200-701 (Korea, Republic of)
- College of Pharmacy, Yeungnam University, Gyongsan 712-749 (Korea, Republic of)
- College of Pharamacy, Sungkyunkwan University, Suwon 440-746 (Korea, Republic of)
- College of Pharmacy, Duksung Women's University, Seoul 132-714 (Korea, Republic of)
The anti-allergic action of various Oriental medicinal herbs was investigated using in vitro and in vivo experimental models. Of these extracts, the ethanol extract of Meliae cortex (MC) exhibited the most potent activity in mast cells; its IC{sub 50} values were 29 {+-} 1.5 {mu}g/ml for antigen stimulation and 57 {+-} 3.4 {mu}g/ml for thapsigargin stimulation. It inhibited compound-48/80-induced systemic anaphylaxis by 52.9% at a dose of 300 mg/kg in mice; it also inhibited the expression of the proinflammatory mediator TNF-{alpha}. With regard to its mechanism of action, MC suppressed the activating phosphorylation of Syk, a key enzyme in mast-cell signaling processes and that of Akt in a dose-dependent manner. It also inhibited the MAP kinase ERK1/2, which is critical for the production of inflammatory cytokines in mast cells, as indicated by the suppression of the activating phosphorylation of ERK1/2. Taken together, these results suggest that the anti-allergic activity of MC may be due to the inhibition of histamine secretion and cytokine expression through the Syk inhibition in mast cells.
- OSTI ID:
- 20976913
- Journal Information:
- Toxicology and Applied Pharmacology, Vol. 220, Issue 3; Other Information: DOI: 10.1016/j.taap.2006.10.034; PII: S0041-008X(06)00391-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0041-008X
- Country of Publication:
- United States
- Language:
- English
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