Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells

Journal Article · Thu Feb 15 00:00:00 EST 2007 · Experimental Cell Research

DOI:https://doi.org/10.1016/j.yexcr.2006.11.012· OSTI ID:20972118

Yamada, Yoji ^[1]; Sakurada, Kazuhiro ^[1]; Takeda, Yukiji ^[2]; Gojo, Satoshi ^[2]; Umezawa, Akihiro ^[2]

---

1. BioFrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533 (Japan)
2. National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535 (Japan)

Bone marrow-derived stromal cells can give rise to cardiomyocytes as well as adipocytes, osteocytes, and chondrocytes in vitro. The existence of mesenchymal stem cells has been proposed, but it remains unclear if a single-cell-derived stem cell stochastically commits toward a cardiac lineage. By single-cell marking, we performed a follow-up study of individual cells during the differentiation of 9-15c mesenchymal stromal cells derived from bone marrow cells. Three types of cells, i.e., cardiac myoblasts, cardiac progenitors and multipotent stem cells were differentiated from a single cell, implying that cardiomyocytes are generated stochastically from a single-cell-derived stem cell. We also demonstrated that overexpression of Csx/Nkx2.5 and GATA4, precardiac mesodermal transcription factors, enhanced cardiomyogenic differentiation of 9-15c cells, and the frequency of cardiomyogenic differentiation was increased by co-culturing with fetal cardiomyocytes. Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 behaved like cardiac transient amplifying cells, and still retained their plasticity in vivo.

• OSTI ID: 20972118
• Journal Information: Experimental Cell Research, Vol. 313, Issue 4; Other Information: DOI: 10.1016/j.yexcr.2006.11.012; PII: S0014-4827(06)00476-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BONE CELLS
BONE MARROW
BONE MARROW CELLS
CARBON 15
IN VITRO
IN VIVO
MYOBLASTS
PLASTICITY
STEM CELLS
TRANSCRIPTION FACTORS
TRANSIENTS