Displacement measurement of the depth migration of transparent cells

Journal Article · Mon Dec 11 00:00:00 EST 2006 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.2405396· OSTI ID:20880157

Yoshida, Makoto; Ishimaru, Ichirou; Ishizaki, Katsumi; Yasokawa, Toshiki; Kuriyama, Shigeki; Masaki, Tsutomu; Nakai, Seiji; Takegawa, Kaoru; Tanaka, Naoyuki ^[1]

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1. Faculty of Engineering, Kagawa University, 2217-20 Hayashi-cho, Takamatsu, Kagawa 761-0396 (Japan)

This letter reports a method for displacement measurement of the depth migration of transparent cells. This proposed optical spatial filtering method allows visualization of the transparent cells and determination of depth migration as a horizontal displacement positive or negative first order diffracted light on the detector surface. When the sample is displaced upward or downward from the focal plane, first and negative first order diffracted light form images at a different point as a light circle. The coordinates of these two light circles on the detector surface change places when the displacement of depth migration moves to the opposite direction.

• OSTI ID: 20880157
• Journal Information: Applied Physics Letters, Vol. 89, Issue 24; Other Information: DOI: 10.1063/1.2405396; (c) 2006 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
BIOPHYSICS
DIFFRACTION
MIGRATION
OPACITY
OPTICAL FILTERS
VISIBLE RADIATION