Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts
- School of Life Science and Biotechnology, Kyungpook National University, Daegu 702-701 (Korea, Republic of)
- CHA Research Institute, Pochon CHA University, Sungnam 463-836 (Korea, Republic of)
- Department of Oral Biochemistry, College of Dentistry, Chonnam National University, Gwangju 500-757 (Korea, Republic of)
- Department of Medical Genetics, College of Medicine, Hanyang University, Seoul 133-791 (Korea, Republic of)
- Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 110-799 (Korea, Republic of)
Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.
- OSTI ID:
- 20854541
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 349, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.08.137; PII: S0006-291X(06)01940-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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