G-rich, a Drosophila selenoprotein, is a Golgi-resident type III membrane protein

Journal Article · Fri Oct 06 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.07.203· OSTI ID:20854501

Chen, Chang Lan ^[1]; Shim, Myoung Sup ^[1]; Chung, Jiyeol ^[1]; Yoo, Hyun-Seung ^[1]; Ha, Ji Min ^[1]; Kim, Jin Young ^[1]; Choi, Jinmi ^[1]; Zang, Shu Liang ^[2]; Hou, Xiao ^[2]; Carlson, Bradley A ^[3]; Hatfield, Dolph L ^[3]; Lee, Byeong Jae ^[1]

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1. School of Biological Sciences and Institute of Molecular Biology and Genetics, Seoul National University, Seoul 151-742 (Korea, Republic of)
2. Academy of Environment and Life Sciences, Liaoning University, Shenyang 110036 (China)
3. Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 (United States)

G-rich is a Drosophila melanogaster selenoprotein, which is a homologue of human and mouse SelK. Subcellular localization analysis using GFP-tagged G-rich showed that G-rich was localized in the Golgi apparatus. The fusion protein was co-localized with the Golgi marker proteins but not with an endoplasmic reticulum (ER) marker protein in Drosophila SL2 cells. Bioinformatic analysis of G-rich suggests that this protein is either type II or type III transmembrane protein. To determine the type of transmembrane protein experimentally, GFP-G-rich in which GFP was tagged at the N-terminus of G-rich, or G-rich-GFP in which GFP was tagged at the C-terminus of G-rich, were expressed in SL2 cells. The tagged proteins were then digested with trypsin, and analyzed by Western blot analysis. The results showed that the C-terminus of the G-rich protein was exposed to the cytoplasm indicating it is a type III microsomal membrane protein. G-rich is First selenoprotein identified in the Golgi apparatus.

• OSTI ID: 20854501
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 348, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.07.203; PII: S0006-291X(06)01742-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CYTOPLASM
DROSOPHILA
ENDOPLASMIC RETICULUM
GOLGI COMPLEXES
MEMBRANE PROTEINS
MICE
SELENIUM
TRYPSIN