A novel type of EWS-CHOP fusion gene in myxoid liposarcoma

Matsui, Yoshito; Ueda, Takafumi; Kubo, Takahiro; Hasegawa, Tadashi; Tomita, Yasuhiko; Okamoto, Mina; Myoui, Akira; Kakunaga, Shigeki; Yasui, Natsuo; Yoshikawa, Hideki

doi:10.1016/j.bbrc.2006.07.085

Title: A novel type of EWS-CHOP fusion gene in myxoid liposarcoma

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Abstract

The cytogenetic hallmark of myxoid type and round cell type liposarcoma consists of reciprocal translocation of t(12;16)(q13;p11) and t(12;22)(q13;q12), which results in fusion of TLS/FUS and CHOP, and EWS and CHOP, respectively. Nine structural variations of the TLS/FUS-CHOP chimeric transcript have been reported, however, only two types of EWS-CHOP have been described. We describe here a case of myxoid liposarcoma containing a novel EWS-CHOP chimeric transcript and identified the breakpoint occurring in intron 13 of EWS. Reverse transcription-polymerase chain reaction and direct sequence showed that exon 13 of EWS was in-frame fused to exon 2 of CHOP. Genomic analysis revealed that the breaks were located in intron 13 of EWS and intron 1 of CHOP.

Authors:

Matsui, Yoshito ^[1]; Ueda, Takafumi ^[1]; Kubo, Takahiro ^[2]; Hasegawa, Tadashi ^[3]; Tomita, Yasuhiko ^[4]; Okamoto, Mina ^[1]; Myoui, Akira ^[1]; Kakunaga, Shigeki ^[1]; Yasui, Natsuo ^[2]; Yoshikawa, Hideki ^[1]

Department of Orthopaedics, Osaka University Graduate School of Medicine (Japan)
Department of Orthopaedics, Institute of the Health Biosciences, University of Tokushima Graduate School (Japan)
Department of Pathology, Sapporo Medical University School of Medicine (Japan)
Department of Pathology, Osaka University Graduate School of Medicine (Japan)

Publication Date:: Fri Sep 22 00:00:00 EDT 2006

OSTI Identifier:: 20854468

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 348; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2006.07.085; PII: S0006-291X(06)01613-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CHROMOSOMAL ABERRATIONS; GENES; POLYMERASE CHAIN REACTION; TRANSCRIPTION; TRANSLOCATION

Citation Formats


                    Matsui, Yoshito, Ueda, Takafumi, Kubo, Takahiro, Hasegawa, Tadashi, Tomita, Yasuhiko, Okamoto, Mina, Myoui, Akira, Kakunaga, Shigeki, Yasui, Natsuo, and Yoshikawa, Hideki. A novel type of EWS-CHOP fusion gene in myxoid liposarcoma.  United States: N. p., 2006. 
        Web.  doi:10.1016/j.bbrc.2006.07.085.

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                    Matsui, Yoshito, Ueda, Takafumi, Kubo, Takahiro, Hasegawa, Tadashi, Tomita, Yasuhiko, Okamoto, Mina, Myoui, Akira, Kakunaga, Shigeki, Yasui, Natsuo, & Yoshikawa, Hideki. A novel type of EWS-CHOP fusion gene in myxoid liposarcoma.  United States.  https://doi.org/10.1016/j.bbrc.2006.07.085

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                    Matsui, Yoshito, Ueda, Takafumi, Kubo, Takahiro, Hasegawa, Tadashi, Tomita, Yasuhiko, Okamoto, Mina, Myoui, Akira, Kakunaga, Shigeki, Yasui, Natsuo, and Yoshikawa, Hideki. 2006.  
        "A novel type of EWS-CHOP fusion gene in myxoid liposarcoma".  United States.  https://doi.org/10.1016/j.bbrc.2006.07.085.

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@article{osti_20854468,

  title        = {A novel type of EWS-CHOP fusion gene in myxoid liposarcoma},

  author       = {Matsui, Yoshito and Ueda, Takafumi and Kubo, Takahiro and Hasegawa, Tadashi and Tomita, Yasuhiko and Okamoto, Mina and Myoui, Akira and Kakunaga, Shigeki and Yasui, Natsuo and Yoshikawa, Hideki},

  abstractNote = {The cytogenetic hallmark of myxoid type and round cell type liposarcoma consists of reciprocal translocation of t(12;16)(q13;p11) and t(12;22)(q13;q12), which results in fusion of TLS/FUS and CHOP, and EWS and CHOP, respectively. Nine structural variations of the TLS/FUS-CHOP chimeric transcript have been reported, however, only two types of EWS-CHOP have been described. We describe here a case of myxoid liposarcoma containing a novel EWS-CHOP chimeric transcript and identified the breakpoint occurring in intron 13 of EWS. Reverse transcription-polymerase chain reaction and direct sequence showed that exon 13 of EWS was in-frame fused to exon 2 of CHOP. Genomic analysis revealed that the breaks were located in intron 13 of EWS and intron 1 of CHOP.},

  doi          = {10.1016/j.bbrc.2006.07.085},

  url          = {https://www.osti.gov/biblio/20854468},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 2,

  volume       = 348,

  place        = {United States},

  year         = {Fri Sep 22 00:00:00 EDT 2006},

  month        = {Fri Sep 22 00:00:00 EDT 2006}

}

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