G protein-coupled receptor 30 is an estrogen receptor in the plasma membrane
- Center for Gene Research, Yamaguchi University, Yamaguchi 755-8505 (Japan)
- Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University School of Medicine, Yamaguchi 755-8505 (Japan)
- Department of Bio-Signal Analysis, AMES, Graduate School of Medicine, Yamaguchi University, Yamaguchi 755-8505 (Japan)
Recently, GPR30 was reported to be a novel estrogen receptor; however, its intracellular localization has remained controversial. To investigate the intracellular localization of GPR30 in vivo, we produced four kinds of polyclonal antibodies for distinct epitopes on GPR30. Immunocytochemical observations using anti-GPR30 antibody and anti-FLAG antibody show that FLAG-GPR30 localizes to the plasma membrane 24 h after transfection. Treatment with estrogen (17{beta}-estradiol or E2) causes an elevation in the intracellular Ca{sup 2+} concentration ([Ca{sup 2+}]{sub i}) within 10 s in HeLa cells expressing FLAG-GPR30. In addition, E2 induces the translocation of GPR30 from the plasma membrane to the cytoplasm by 1 h after stimulation. Immunohistochemical analysis shows that GPR30 exists on the cell surface of CA2 pyramidal neuronal cells. The images on transmission electron microscopy show that GPR30 is localized to a particular region associated with the plasma membranes of the pyramidal cells. These data indicate that GPR30, a transmembrane receptor for estrogen, is localized to the plasma membrane of CA2 pyramidal neuronal cells of the hippocampus in rat brain.
- OSTI ID:
- 20854392
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 346, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.05.191; PII: S0006-291X(06)01279-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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