Arginine-rich intracellular delivery peptides noncovalently transport protein into living cells

Journal Article · Fri Aug 04 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.05.205· OSTI ID:20854388

Wang, Y -H ^[1]; Chen, C -P ^[1]; Chan, M -H ^[2]; Chang, M ^[1]; Hou, Y -W ^[1]; Chen, H -H ^[2]; Hsu, H -R ^[2]; Liu, Kevin ^[1]; Lee, H -J ^[1]

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1. Department of Life Science, National Dong Hwa University, Hualien 97401, Taiwan (China)
2. Institute of Pharmacology and Toxicology, Tzu Chi University, Hualien 97004, Taiwan (China)

Plasma membranes of plant or animal cells are generally impermeable to peptides or proteins. Many basic peptides have previously been investigated and covalently cross-linked with cargoes for cellular internalization. In the current study, we demonstrate that arginine-rich intracellular delivery (AID) peptides are able to deliver fluorescent proteins or {beta}-galactosidase enzyme into animal and plant cells, as well as animal tissue. Cellular internalization and transdermal delivery of protein could be mediated by effective and nontoxic AID peptides in a neither fusion protein nor conjugation fashion. Therefore, noncovalent AID peptides may provide a useful strategy to have active proteins function in living cells and tissues in vivo.

• OSTI ID: 20854388
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 346, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.05.205; PII: S0006-291X(06)01238-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ARGININE
GALACTOSIDASE
IN VIVO
PEPTIDES
PLANT CELLS