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Title: Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens

Abstract

From the dried fruiting bodies of the mushroom Armillaria luteo-virens, a dimeric lectin with a molecular mass of 29.4 kDa has been isolated. The purification procedure involved (NH{sub 4}){sub 2}SO{sub 4} precipitation, ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The hemagglutinating activity of the lectin could not be inhibited by simple sugars but was inhibited by the polysaccharide inulin. The activity was stable up to 70 {sup o}C but was acid- and alkali-labile. Salts including FeCl{sub 3}, AlCl{sub 3}, and ZnCl{sub 2} inhibited the activity whereas MgCl{sub 2}, MnCl{sub 2}, and CaCl{sub 2} did not. The lectin stimulated mitogenic response of mouse splenocytes with the maximal response achieved by 1 {mu}M lectin. Proliferation of tumor cells including MBL2 cells, HeLa cells, and L1210 cells was inhibited by the lectin with an IC{sub 5} of 2.5, 5, and 10 {mu}M, respectively. However, proliferation of HepG2 cells was not affected. The novel aspects of the isolated lectin include a novel N-terminal sequence, fair thermostability, acid stability, and alkali stability, together with potent mitogenic activity toward spleen cells and antiproliferative activity toward tumor cells.

Authors:
 [1];  [1];  [2];  [1];  [3];  [3];  [3];  [3];  [1]
  1. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094 (China)
  2. Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, New Territories, Hong Kong (China)
  3. Agriculture and Animal Husbandry College, Qinghai University, Xining 810003 (China)
Publication Date:
OSTI Identifier:
20854360
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 345; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.05.061; PII: S0006-291X(06)01106-5; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMMONIUM SULFATES; CALCIUM CHLORIDES; CELL PROLIFERATION; CELLULOSE; GELS; HELA CELLS; INULIN; ION EXCHANGE CHROMATOGRAPHY; IRON CHLORIDES; LIQUID COLUMN CHROMATOGRAPHY; MICE; MUSHROOMS; PRECIPITATION; PROTEINS; SACCHAROSE; SPLEEN CELLS; ZINC CHLORIDES

Citation Formats

Feng, K, College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, Liu, Q H, Ng, T B, Liu, H Z, Li, J Q, Chen, G, Sheng, H Y, Xie, Z L, and Wang, H X. Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.05.061.
Feng, K, College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, Liu, Q H, Ng, T B, Liu, H Z, Li, J Q, Chen, G, Sheng, H Y, Xie, Z L, & Wang, H X. Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens. United States. https://doi.org/10.1016/j.bbrc.2006.05.061
Feng, K, College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, Liu, Q H, Ng, T B, Liu, H Z, Li, J Q, Chen, G, Sheng, H Y, Xie, Z L, and Wang, H X. 2006. "Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens". United States. https://doi.org/10.1016/j.bbrc.2006.05.061.
@article{osti_20854360,
title = {Isolation and characterization of a novel lectin from the mushroom Armillaria luteo-virens},
author = {Feng, K and College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319 and Liu, Q H and Ng, T B and Liu, H Z and Li, J Q and Chen, G and Sheng, H Y and Xie, Z L and Wang, H X},
abstractNote = {From the dried fruiting bodies of the mushroom Armillaria luteo-virens, a dimeric lectin with a molecular mass of 29.4 kDa has been isolated. The purification procedure involved (NH{sub 4}){sub 2}SO{sub 4} precipitation, ion exchange chromatography on DEAE-cellulose, CM-cellulose, and Q-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The hemagglutinating activity of the lectin could not be inhibited by simple sugars but was inhibited by the polysaccharide inulin. The activity was stable up to 70 {sup o}C but was acid- and alkali-labile. Salts including FeCl{sub 3}, AlCl{sub 3}, and ZnCl{sub 2} inhibited the activity whereas MgCl{sub 2}, MnCl{sub 2}, and CaCl{sub 2} did not. The lectin stimulated mitogenic response of mouse splenocytes with the maximal response achieved by 1 {mu}M lectin. Proliferation of tumor cells including MBL2 cells, HeLa cells, and L1210 cells was inhibited by the lectin with an IC{sub 5} of 2.5, 5, and 10 {mu}M, respectively. However, proliferation of HepG2 cells was not affected. The novel aspects of the isolated lectin include a novel N-terminal sequence, fair thermostability, acid stability, and alkali stability, together with potent mitogenic activity toward spleen cells and antiproliferative activity toward tumor cells.},
doi = {10.1016/j.bbrc.2006.05.061},
url = {https://www.osti.gov/biblio/20854360}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 345,
place = {United States},
year = {Fri Jul 14 00:00:00 EDT 2006},
month = {Fri Jul 14 00:00:00 EDT 2006}
}