Establishment of primary cultures for mouse ameloblasts as a model of their lifetime
- Department of Biochemistry, School of Dentistry, Showa University, Tokyo 142-8555 (Japan)
- Institute for Oral Science, Matsumoto Dental University, Shiojiri 399-0781 (Japan)
- Division of Pathophysiology, Research Center for Genomic Medicine, Saitama Medical School, Hidaka 350-1241 (Japan)
- Department of Clinical Pharmacy, School of Pharmaceutical Science, Showa University (Japan)
To understand how the properties of ameloblasts are spatiotemporally regulated during amelogenesis, two primary cultures of ameloblasts in different stages of differentiation were established from mouse enamel epithelium. Mouse primary ameloblasts (MPAs) prepared from immature enamel epithelium (MPA-I) could proliferate, whereas those from mature enamel epithelium (MPA-M) could not. MPA-M but not MPA-I caused apoptosis during culture. The mRNA expression of amelogenin, a marker of immature ameloblasts, was down-regulated, and that of enamel matrix serine proteiase-1, a marker of mature ameloblasts, was induced in MPA-I during culture. Using green fluorescence protein as a reporter, a visualized reporter system was established to analyze the promoter activity of the amelogenin gene. The region between -1102 bp and -261 bp was required for the reporter expression in MPA-I. These results suggest that MPAs are valuable in vitro models for investigation of ameloblast biology, and that the visualized system is useful for promoter analysis in MPAs.
- OSTI ID:
- 20854347
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 345, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.04.122; PII: S0006-291X(06)00932-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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