Rabies virus glycoprotein as a carrier for anthrax protective antigen

Journal Article · Sat Sep 30 00:00:00 EDT 2006 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.05.010· OSTI ID:20850566

Smith, Mary Ellen ^[1]; Koser, Martin ^[1]; Sa, Xiao ^[2]; Siler, Catherine ^[3]; McGettigan, James P ^[1]; Calkins, Catherine ^[1]; Pomerantz, Roger J ^[3]; Dietzschold, Bernhard ^[1]; Schnell, Matthias J ^[4]

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1. Departments of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)
2. Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029 (United States)
3. Departments of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)
4. Departments of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA 19107 (United States) and Departments of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107 (United States)

Live viral vectors expressing foreign antigens have shown great promise as vaccines against viral diseases. However, safety concerns remain a major problem regarding the use of even highly attenuated viral vectors. Using the rabies virus (RV) envelope protein as a carrier molecule, we show here that inactivated RV particles can be utilized to present Bacillus anthracis protective antigen (PA) domain-4 in the viral membrane. In addition to the RV glycoprotein (G) transmembrane and cytoplasmic domains, a portion of the RV G ectodomain was required to express the chimeric RV G anthrax PA on the cell surface. The novel antigen was also efficiently incorporated into RV virions. Mice immunized with the inactivated recombinant RV virions exhibited seroconversion against both RV G and anthrax PA, and a second inoculation greatly increased these responses. These data demonstrate that a viral envelope protein can carry a bacterial protein and that a viral carrier can display whole polypeptides compared to the limited epitope presentation of previous viral systems.

• OSTI ID: 20850566
• Journal Information: Virology, Vol. 353, Issue 2; Other Information: DOI: 10.1016/j.virol.2006.05.010; PII: S0042-6822(06)00331-X; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIGENS
BACILLUS
DISEASE VECTORS
GLYCOPROTEINS
INOCULATION
MICE
POLYPEPTIDES
RABIES
VACCINES
VIRUSES