The pathogenesis of Newcastle disease: A comparison of selected Newcastle disease virus wild-type strains and their infectious clones
- Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, GA 30605 (United States)
- Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605 (United States)
- Poultry Microbiological Safety Unit, Russell Research Center, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605 (United States)
- Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, MD 20742 (United States)
The effect of mutations of Newcastle disease virus (NDV) fusion (F) gene, hemagglutinin-neuraminidase (HN) gene, and phosphoprotein (P) gene and HN chimeras between the virulent Beaudette C and low virulence LaSota strains on pathogenesis and pathogenicity was examined in fully susceptible chickens. A virulent F cleavage site motif within a LaSota backbone increased pathogenicity and severity of clinical disease. A LaSota HN within a Beaudette C backbone decreased pathogenicity indices and disease severity. A Beaudette C HN within a LaSota backbone did not change either pathogenicity indices or severity of disease in chickens. Loss of glycosylation at site 4 of the HN or modified P gene of Beaudette C decreased pathogenicity indices and caused no overt clinicopathologic disease in chickens. Both pathogenicity indices and clinicopathologic examination demonstrated that the F, HN, and P genes of NDV collectively or individually can contribute to viral virulence.
- OSTI ID:
- 20850565
- Journal Information:
- Virology, Vol. 353, Issue 2; Other Information: DOI: 10.1016/j.virol.2006.06.013; PII: S0042-6822(06)00409-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
- Country of Publication:
- United States
- Language:
- English
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