Dual regulation of the {delta}Np63 transcriptional activity by {delta}Np63 in human nasopharyngeal carcinoma cell
- Department of Physiology, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan (China)
- Center of General Study, Chang Gung University, Taoyuan 333, Taiwan (China)
p63 splicing variants lacking NH{sub 2}-terminal transactivating domain, known as {delta}Np63, are thought to antagonize p53 and p63 functions and are suggested to play roles in keratinocyte differentiation. Here, we show that {delta}Np63 has a dual-regulatory effect on the activity of its own promoter in NPC-076 cell. Down-regulation of the transcriptional activity is observed when {delta}Np63 is present in low levels. In contrast, up-regulation of {delta}Np63 transcriptional activity is observed when {delta}Np63 is expressed at higher levels. The down-regulation effect is abolished when the p53-binding site of the {delta}Np63 promoter is mutated. In sharp contrast, similar mutation does not affect the up-regulation of the {delta}Np63 transcriptional activity under the same experimental conditions. Further study shows that the up-regulation is correlated with the activation of the STAT3, as the blockade of STAT3 nuclear translocation abolishes the up-regulation by {delta}Np63. Thus, {delta}Np63 exerts a bidirectional regulation of the {delta}Np63 transcriptional activity in NPC-076 cell.
- OSTI ID:
- 20798912
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 342, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2006.02.111; PII: S0006-291X(06)00411-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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