Involvement of VAMP-2 in exocytosis of IL-1{beta} in turbot (Scophthalmus maximus) leukocytes after Vibrio anguillarum infection

Journal Article · Fri Apr 07 00:00:00 EDT 2006 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2006.01.138· OSTI ID:20798881

Yingmei, Chai ^[1]; Xiaohang, Huang ^[2]; Bailin, Cong ^[2]; Shenghao, Liu ^[2]; Kui, Chen ^[2]; Guangyou, Li ^[2]; Gaisano, Herbert Y ^[3]

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1. Department of Life Science, Ocean University of China, Qingdao 266003 (China)
2. Marine Bioactive Substances Laboratory, First Institute of Oceanography, SOA, Qingdao 266061 (China)
3. Department of Medicine, University of Toronto, Toronto, M5S 1A8 (Canada)

Vibrio anguillarum is a major pathogen threatening the fish aquaculture in China. Infection of cultivated turbot (Scophthalmus maximus) with V. anguillarum induced rapid synthesis and secretion of IL-1{beta}, which initiates the innate immune response. SNARE proteins are known to regulate vesicular trafficking and fusion in all eukaryotes. Here, we determined whether SNARE proteins, specifically vesicle-associated membrane protein-2 (VAMP-2), are involved in regulated exocytosis of IL-1{beta} of leukocytes in marine fish. We show that VAMP-2 is present in turbot blood leukocytes, with nucleotide sequence identity of 88.2% and 93.0% to those of zebra fish and sea bass, respectively. After Vibrio infection, turbot leukocyte VAMP-2 was increased at the levels of transcription and translation in a temporal pattern coinciding with leukocyte IL-1{beta} secretion. Confocal microscopy localized VAMP-2 to vesicle structures in leukocytes. Taken together, our results suggest that VAMP-2 is involved in regulated exocytosis of cytokines in immunocytes in fish.

• OSTI ID: 20798881
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 342, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2006.01.138; PII: S0006-291X(06)00233-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AQUACULTURE
BIOSYNTHESIS
LEUKOCYTES
LYMPHOKINES
MEMBRANE PROTEINS
MICROSCOPY
NUCLEOTIDES
PATHOGENS
SECRETION
TRANSCRIPTION