Subcellular localization and regulation of type-1C and type-5 phosphodiesterases
- Department of Public Health and Cellular Biology, 2nd University of Rome 'Tor Vergata', Rome (Italy)
- Department of Basic and Applied Biology, University of L'Aquila, L'Aquila (Italy)
- Department of Experimental Medicine, University of L'Aquila, L'Aquila (Italy)
- Department of Surgery, 2nd University of Rome 'Tor Vergata', Rome (Italy)
- Department of Medical Pathophysiology, 1st University of Rome 'La Sapienza', Rome (Italy)
We investigated the subcellular localization of PDE5 in in vitro human myometrial cells. We demonstrated for First time that PDE5 is localized in discrete cytoplasmic foci and vesicular compartments corresponding to centrosomes. We also found that PDE5 intracellular localization is not cell- or species-specific, as it is conserved in different animal and human cells. PDE5 protein levels are strongly regulated by the mitotic activity of the smooth muscle cells (SMCs), as they were increased in quiescent, contractile myometrial cultures, and conditions in which proliferation was inhibited. In contrast, PDE1C levels decreased in all conditions that inhibited proliferation. This mirrored the enzymatic activity of both PDE5 and PDE1C. Increasing cGMP intracellular levels by dbcGMP or sildenafil treatments did not block proliferation, while dbcAMP inhibited myometrial cell proliferation. Together, these results suggest that PDE5 regulation of cGMP intracellular levels is not involved in the control of SMC cycle progression, but may represent one of the markers of the contractile phenotype.
- OSTI ID:
- 20798845
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 341, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2006.01.035; PII: S0006-291X(06)00056-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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