Clonal proliferation of multipotent stem/progenitor cells in the neonatal and adult salivary glands
- Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University, Yokohama 236-0004 (Japan)
- Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Yokohama City University, Yokohama 236-0004 (Japan)
- Department of Regenerative Medicine, Graduate School of Medicine, Yokohama City University, Yokohama 236-0004 (Japan) and Research Unit for Organ Regeneration, Center for Developmental Biology, RIKEN, Kobe 650-0047 (Japan) and Biomaterials Center, National Institute for Materials Science, Tsukuba 305-0044 (Japan)
Salivary gland stem/progenitor cells are thought to be present in intercalated ductal cells, but the fact is unclear. In this study, we sought to clarify if stem/progenitor cells are present in submandibular glands using colony assay, which is one of the stem cell assay methods. Using a low-density culture of submandibular gland cells of neonatal rats, we developed a novel culture system that promotes single cell colony formation. Average doubling time for the colony-forming cells was 24.7 (SD = {+-}7.02) h, indicating high proliferative potency. When epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were added to the medium, the number of clonal colonies increased greater than those cultured without growth factors (13.2 {+-} 4.18 vs. 4.5 {+-} 1.73). The RT-PCR and immunostaining demonstrated expressing acinar, ductal, and myoepithelial cell lineage markers. This study demonstrated the presence of the salivary gland stem/progenitor cells that are highly proliferative and multipotent in salivary glands.
- OSTI ID:
- 20798794
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 340, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2005.12.031; PII: S0006-291X(05)02748-8; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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