Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

Kishigami, Satoshi; Mizutani, Eiji; Ohta, Hiroshi; Hikichi, Takafusa; Thuan, Nguyen Van; Wakayama, Sayaka; Bui, Hong-Thuy; Wakayama, Teruhiko

doi:10.1016/j.bbrc.2005.11.164

Title: Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer

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Abstract

The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably reduce abnormal DNA hypermethylation depending on the origins of transferred nuclei and their genomic regions [S. Kishigami, N. Van Thuan, T. Hikichi, H. Ohta, S. Wakayama. E. Mizutani, T. Wakayama, Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids, Dev. Biol. (2005) in press]. Here, we found that 5-50 nM TSA-treatment for 10 h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells, spleen cells, neural stem cells, and cumulus cells. This TSA-treatment also led to more than 5-fold increase in success rate of mouse cloning from cumulus cells without obvious abnormality but failed to improve ES cloning success. Further, we succeeded in establishment of nuclear transfer-embryonic stem (NT-ES) cells from TSA-treated cloned blastocyst at a rate three times higher than those frommore »« less

Authors:

Kishigami, Satoshi ^[1]; Mizutani, Eiji ^[1]; Ohta, Hiroshi ^[1]; Hikichi, Takafusa ^[1]; Thuan, Nguyen Van ^[1]; Wakayama, Sayaka ^[1]; Bui, Hong-Thuy ^[1]; Wakayama, Teruhiko ^[1]

Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, 2-2-3 Minatojima-minamimachi, Kobe 650-0047 (Japan)

Publication Date:: Fri Feb 03 00:00:00 EST 2006

OSTI Identifier:: 20798781

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 340; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.11.164; PII: S0006-291X(05)02710-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; CELL NUCLEI; CLONING; DNA; ERRORS; IN VITRO; MICE; OOCYTES; SPERMATOZOA; SPLEEN CELLS; STEM CELLS; ZYGOTES

Citation Formats


                    Kishigami, Satoshi, Mizutani, Eiji, Ohta, Hiroshi, Hikichi, Takafusa, Thuan, Nguyen Van, Wakayama, Sayaka, Bui, Hong-Thuy, and Wakayama, Teruhiko. Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.  United States: N. p., 2006. 
        Web.  doi:10.1016/j.bbrc.2005.11.164.

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                    Kishigami, Satoshi, Mizutani, Eiji, Ohta, Hiroshi, Hikichi, Takafusa, Thuan, Nguyen Van, Wakayama, Sayaka, Bui, Hong-Thuy, & Wakayama, Teruhiko. Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.  United States.  https://doi.org/10.1016/j.bbrc.2005.11.164

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                    Kishigami, Satoshi, Mizutani, Eiji, Ohta, Hiroshi, Hikichi, Takafusa, Thuan, Nguyen Van, Wakayama, Sayaka, Bui, Hong-Thuy, and Wakayama, Teruhiko. 2006.  
        "Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer".  United States.  https://doi.org/10.1016/j.bbrc.2005.11.164.

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@article{osti_20798781,

  title        = {Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer},

  author       = {Kishigami, Satoshi and Mizutani, Eiji and Ohta, Hiroshi and Hikichi, Takafusa and Thuan, Nguyen Van and Wakayama, Sayaka and Bui, Hong-Thuy and Wakayama, Teruhiko},

  abstractNote = {The low success rate of animal cloning by somatic cell nuclear transfer (SCNT) is believed to be associated with epigenetic errors including abnormal DNA hypermethylation. Recently, we elucidated by using round spermatids that, after nuclear transfer, treatment of zygotes with trichostatin A (TSA), an inhibitor of histone deacetylase, can remarkably reduce abnormal DNA hypermethylation depending on the origins of transferred nuclei and their genomic regions [S. Kishigami, N. Van Thuan, T. Hikichi, H. Ohta, S. Wakayama. E. Mizutani, T. Wakayama, Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids, Dev. Biol. (2005) in press]. Here, we found that 5-50 nM TSA-treatment for 10 h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells, spleen cells, neural stem cells, and cumulus cells. This TSA-treatment also led to more than 5-fold increase in success rate of mouse cloning from cumulus cells without obvious abnormality but failed to improve ES cloning success. Further, we succeeded in establishment of nuclear transfer-embryonic stem (NT-ES) cells from TSA-treated cloned blastocyst at a rate three times higher than those from untreated cloned blastocysts. Thus, our data indicate that TSA-treatment after SCNT in mice can dramatically improve the practical application of current cloning techniques.},

  doi          = {10.1016/j.bbrc.2005.11.164},

  url          = {https://www.osti.gov/biblio/20798781},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 1,

  volume       = 340,

  place        = {United States},

  year         = {Fri Feb 03 00:00:00 EST 2006},

  month        = {Fri Feb 03 00:00:00 EST 2006}

}

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