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Title: Identification of a novel male germ cell-specific gene TESF-1 in mice

Abstract

Mammalian spermatogenesis is precisely regulated by many germ cell-specific factors. In search for such a germ cell-specific factor, we have identified a novel mouse gene testis-specific factor 1 (TESF-1). Messenger RNA of TESF-1 was found only in the testis and its expression appeared to be regulated in a developmental manner. Further analysis demonstrated that the expression of TESF-1 was specifically in male germ cells, supported by the observation that we were not able to detect the TESF-1 mRNA from at/at homozygous mutant testes, which lack germ cells. The deduced amino acid sequence of TESF-1 contains a leucine-zipper motif, a potential nuclear localization signal, and two cAMP- and cGMP-dependent protein kinase phosphorylation sites. The green fluorescent protein (GFP)-tagged TESF-1 fusion protein was expressed in COS-7 cells and localized primarily in the nucleus. Taken together, these results indicate that TESF-1 is a novel male germ cell-specific gene, and its protein product may function as a nuclear factor involved in the regulation of spermatogenesis.

Authors:
 [1];  [1];  [1];  [2];  [1]
  1. Department of Biological Sciences, Marshall University, Huntington, WV (United States)
  2. Department of Anatomy and Pathology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV (United States)
Publication Date:
OSTI Identifier:
20798771
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 340; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.11.152; PII: S0006-291X(05)02669-0; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; AMP; GENE REGULATION; GENES; GERM CELLS; LEUCINE; MESSENGER-RNA; MICE; MUTANTS; PHOSPHORYLATION; PROTEINS; SPERMATOGENESIS; TESTES

Citation Formats

Jun, Fan, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L, and Guozhang, Zhu. Identification of a novel male germ cell-specific gene TESF-1 in mice. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.11.152.
Jun, Fan, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L, & Guozhang, Zhu. Identification of a novel male germ cell-specific gene TESF-1 in mice. United States. https://doi.org/10.1016/j.bbrc.2005.11.152
Jun, Fan, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L, and Guozhang, Zhu. 2006. "Identification of a novel male germ cell-specific gene TESF-1 in mice". United States. https://doi.org/10.1016/j.bbrc.2005.11.152.
@article{osti_20798771,
title = {Identification of a novel male germ cell-specific gene TESF-1 in mice},
author = {Jun, Fan and Graham, Matthew and Akabane, Hiroto and Richardson, Laura L and Guozhang, Zhu},
abstractNote = {Mammalian spermatogenesis is precisely regulated by many germ cell-specific factors. In search for such a germ cell-specific factor, we have identified a novel mouse gene testis-specific factor 1 (TESF-1). Messenger RNA of TESF-1 was found only in the testis and its expression appeared to be regulated in a developmental manner. Further analysis demonstrated that the expression of TESF-1 was specifically in male germ cells, supported by the observation that we were not able to detect the TESF-1 mRNA from at/at homozygous mutant testes, which lack germ cells. The deduced amino acid sequence of TESF-1 contains a leucine-zipper motif, a potential nuclear localization signal, and two cAMP- and cGMP-dependent protein kinase phosphorylation sites. The green fluorescent protein (GFP)-tagged TESF-1 fusion protein was expressed in COS-7 cells and localized primarily in the nucleus. Taken together, these results indicate that TESF-1 is a novel male germ cell-specific gene, and its protein product may function as a nuclear factor involved in the regulation of spermatogenesis.},
doi = {10.1016/j.bbrc.2005.11.152},
url = {https://www.osti.gov/biblio/20798771}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 340,
place = {United States},
year = {Fri Feb 03 00:00:00 EST 2006},
month = {Fri Feb 03 00:00:00 EST 2006}
}