Negatively charged phospholipids suppress IFN-{gamma} production in T cells
- School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan)
- Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo 143-8540 (Japan)
The effect of phospholipids on IFN-{gamma} production in mouse T cells was investigated. Phosphatidylserine (PS), which has a negatively charged head group, completely inhibited IFN-{gamma} production in splenic naive T cells and antigen-dependent IFN-{gamma} production in Th1 clone 42-6A cells, whereas other phospholipids, which have neutrally charged head group, had no effect. The structural requirements for IFN-{gamma} inhibitory effects by PS were investigated, and dimyristoyl-PS (C14: 0) and dipalmitoyl-PS (C16: 0) had no effect on IFN-{gamma} production, and interestingly, distearoyl-PS (18: 0) increased IFN-{gamma} production. Dioleoyl-PS (C18: 1), dilinoleoyl-PS (C18: 2), and oleoyl-lyso-PS (C18: 1) completely inhibited IFN-{gamma} production. To clarify this mechanism, we focused on the stability of IFN-{gamma} mRNA, and the treatment of splenic naive T cells with PS brought about 40% reductions in IFN-{gamma} mRNA expression in the presence of actinomycin D. Collectively, IFN-{gamma} inhibitory effects by PS are highly dependent on the molecular structure of PS and involve the decreasing of the stability of IFN-{gamma} mRNA.
- OSTI ID:
- 20793241
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 338, Issue 4; Other Information: DOI: 10.1016/j.bbrc.2005.10.179; PII: S0006-291X(05)02481-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression of cocoa genes in Saccharomyces cerevisiae improves cocoa butter production
Activation of phospholipase C in human neutrophils (PMN) by phorbol myristate acetate (PMA)