Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor

Raymond, Marc-Andre; Desormeaux, Anik; Labelle, Andree; Soulez, Mathilde; Soulez, Gilles; Langelier, Yves; Pshezhetsky, Alexey V; Hebert, Marie-Josee

doi:10.1016/J.BBRC.2005.1

Title: Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor

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Abstract

Endothelial cells (EC) under stress release paracrine mediators that facilitate accumulation of vascular smooth muscle cells (VSCM) at sites of vascular injury. We found that medium conditioned by serum-starved EC increase proliferation and migration of VSCM in vitro. Fractionation of the conditioned medium followed by mass spectral analysis identified one bioactive component as vitamin D-binding protein (DBP). DBP induced both proliferation and migration of VSMC in vitro in association with increased phosphorylation of ERK 1/2. PD 98059, a biochemical inhibitor of ERK 1/2, abrogated these proliferative and migratory responses in VSMC. DBP is an important carrier for the vitamin-D sterols, 25-hydroxyvitamin-D, and 1{alpha},25-dihydroxyvitamin-D. Both sterols inhibited the activity of DBP on VSMC, suggesting that vitamin D binding sites are important for initiating the activities of DBP on VSMC. Release of DBP at sites of endothelial injury represents a novel pathway favoring accumulation of VSMC at sites of vascular injury.

Authors:

Raymond, Marc-Andre ^[1]; Desormeaux, Anik ^[1]; Labelle, Andree ^[1]; Soulez, Mathilde ^[1]; Soulez, Gilles ^[1]; Langelier, Yves ^[1]; Pshezhetsky, Alexey V ^[2]; Hebert, Marie-Josee ^[1]

Research Centre CHUM, University of Montreal, 1560 Sherbrooke East, Montreal, Que., H2L 4M1 (Canada)
Ste-Justine Hospital Research Centre, University of Montreal, 3175 Cote Ste-Catherine, Montreal, Que., H3T 1C5 (Canada)

Publication Date:: Fri Dec 23 00:00:00 EST 2005

OSTI Identifier:: 20793234

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 338; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2005.10.105; PII: S0006-291X(05)02323-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; BIOLOGICAL STRESS; CELL PROLIFERATION; DBP; GROWTH FACTORS; INJURIES; MUSCLES; PHOSPHORYLATION; STEROLS; VITAMIN D

Citation Formats


                    Raymond, Marc-Andre, Desormeaux, Anik, Labelle, Andree, Soulez, Mathilde, Soulez, Gilles, Langelier, Yves, Pshezhetsky, Alexey V, and Hebert, Marie-Josee. Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor.  United States: N. p., 2005. 
        Web.  doi:10.1016/J.BBRC.2005.1.

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                    Raymond, Marc-Andre, Desormeaux, Anik, Labelle, Andree, Soulez, Mathilde, Soulez, Gilles, Langelier, Yves, Pshezhetsky, Alexey V, & Hebert, Marie-Josee. Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor.  United States.  https://doi.org/10.1016/J.BBRC.2005.1

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                    Raymond, Marc-Andre, Desormeaux, Anik, Labelle, Andree, Soulez, Mathilde, Soulez, Gilles, Langelier, Yves, Pshezhetsky, Alexey V, and Hebert, Marie-Josee. 2005.  
        "Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor".  United States.  https://doi.org/10.1016/J.BBRC.2005.1.

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@article{osti_20793234,

  title        = {Endothelial stress induces the release of vitamin D-binding protein, a novel growth factor},

  author       = {Raymond, Marc-Andre and Desormeaux, Anik and Labelle, Andree and Soulez, Mathilde and Soulez, Gilles and Langelier, Yves and Pshezhetsky, Alexey V and Hebert, Marie-Josee},

  abstractNote = {Endothelial cells (EC) under stress release paracrine mediators that facilitate accumulation of vascular smooth muscle cells (VSCM) at sites of vascular injury. We found that medium conditioned by serum-starved EC increase proliferation and migration of VSCM in vitro. Fractionation of the conditioned medium followed by mass spectral analysis identified one bioactive component as vitamin D-binding protein (DBP). DBP induced both proliferation and migration of VSMC in vitro in association with increased phosphorylation of ERK 1/2. PD 98059, a biochemical inhibitor of ERK 1/2, abrogated these proliferative and migratory responses in VSMC. DBP is an important carrier for the vitamin-D sterols, 25-hydroxyvitamin-D, and 1{alpha},25-dihydroxyvitamin-D. Both sterols inhibited the activity of DBP on VSMC, suggesting that vitamin D binding sites are important for initiating the activities of DBP on VSMC. Release of DBP at sites of endothelial injury represents a novel pathway favoring accumulation of VSMC at sites of vascular injury.},

  doi          = {10.1016/J.BBRC.2005.1},

  url          = {https://www.osti.gov/biblio/20793234},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 3,

  volume       = 338,

  place        = {United States},

  year         = {Fri Dec 23 00:00:00 EST 2005},

  month        = {Fri Dec 23 00:00:00 EST 2005}

}

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