Calibrating bead displacements in optical tweezers using acousto-optic deflectors

Vermeulen, Karen C; Mameren, Joost van; Stienen, Ger J.M.; Peterman, Erwin J.G.; Wuite, Gijs J.L.; Schmidt, Christoph F

doi:10.1063/1.2165568

Title: Calibrating bead displacements in optical tweezers using acousto-optic deflectors

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Abstract

Displacements of optically trapped particles are often recorded using back-focal-plane interferometry. In order to calibrate the detector signals to displacements of the trapped object, several approaches are available. One often relies either on scanning a fixed bead across the waist of the laser beam or on analyzing the power spectrum of movements of the trapped bead. Here, we introduce an alternative method to perform this calibration. The method consists of very rapidly scanning the laser beam across the solvent-immersed, trapped bead using acousto-optic deflectors while recording the detector signals. It does not require any knowledge of solvent viscosity and bead diameter, and works in all types of samples, viscous or viscoelastic. Moreover, it is performed with the same bead as that used in the actual experiment. This represents marked advantages over established methods.

Authors:

Vermeulen, Karen C; Mameren, Joost van; Stienen, Ger J.M.; Peterman, Erwin J.G.; Wuite, Gijs J.L.; Schmidt, Christoph F ^[1]

Laser Centre and Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam (Netherlands)

Publication Date:: Sun Jan 15 00:00:00 EST 2006

OSTI Identifier:: 20778562

Resource Type:: Journal Article

Journal Name:: Review of Scientific Instruments

Additional Journal Information:: Journal Volume: 77; Journal Issue: 1; Other Information: DOI: 10.1063/1.2165568; (c) 2006 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0034-6748

Country of Publication:: United States

Language:: English

Subject:: 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; BEAMS; CALIBRATION; INTERFEROMETRY; LASERS; RADIATION PRESSURE; SIGNALS; TRAPPING; VISCOSITY

Citation Formats


                    Vermeulen, Karen C, Mameren, Joost van, Stienen, Ger J.M., Peterman, Erwin J.G., Wuite, Gijs J.L., Schmidt, Christoph F, Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, and Laser Centre and Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam. Calibrating bead displacements in optical tweezers using acousto-optic deflectors.  United States: N. p., 2006. 
        Web.  doi:10.1063/1.2165568.

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                    Vermeulen, Karen C, Mameren, Joost van, Stienen, Ger J.M., Peterman, Erwin J.G., Wuite, Gijs J.L., Schmidt, Christoph F, Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, & Laser Centre and Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam. Calibrating bead displacements in optical tweezers using acousto-optic deflectors.  United States.  https://doi.org/10.1063/1.2165568

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                    Vermeulen, Karen C, Mameren, Joost van, Stienen, Ger J.M., Peterman, Erwin J.G., Wuite, Gijs J.L., Schmidt, Christoph F, Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, and Laser Centre and Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam. 2006.  
        "Calibrating bead displacements in optical tweezers using acousto-optic deflectors".  United States.  https://doi.org/10.1063/1.2165568.

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@article{osti_20778562,

  title        = {Calibrating bead displacements in optical tweezers using acousto-optic deflectors},

  author       = {Vermeulen, Karen C and Mameren, Joost van and Stienen, Ger J.M. and Peterman, Erwin J.G. and Wuite, Gijs J.L. and Schmidt, Christoph F and Laboratory for Physiology, Institute for Cardiovascular Research, VU Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam and Laser Centre and Department of Physics and Astronomy, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam},

  abstractNote = {Displacements of optically trapped particles are often recorded using back-focal-plane interferometry. In order to calibrate the detector signals to displacements of the trapped object, several approaches are available. One often relies either on scanning a fixed bead across the waist of the laser beam or on analyzing the power spectrum of movements of the trapped bead. Here, we introduce an alternative method to perform this calibration. The method consists of very rapidly scanning the laser beam across the solvent-immersed, trapped bead using acousto-optic deflectors while recording the detector signals. It does not require any knowledge of solvent viscosity and bead diameter, and works in all types of samples, viscous or viscoelastic. Moreover, it is performed with the same bead as that used in the actual experiment. This represents marked advantages over established methods.},

  doi          = {10.1063/1.2165568},

  url          = {https://www.osti.gov/biblio/20778562},
  journal      = {Review of Scientific Instruments},

  issn         = {0034-6748},

  number       = 1,

  volume       = 77,

  place        = {United States},

  year         = {Sun Jan 15 00:00:00 EST 2006},

  month        = {Sun Jan 15 00:00:00 EST 2006}

}

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