Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression
Abstract
We demonstrate a cell extract-based, genome-wide and heritable reprogramming of gene expression in vitro. Kidney epithelial 293T cells have previously been shown to take on T cell properties following a brief treatment with an extract of Jurkat T cells. We show here that 293T cells exposed for 1 h to a Jurkat cell extract undergo genome-wide, target cell-type-specific and long-lasting transcriptional changes. Microarray analyses indicate that on any given week after extract treatment, {approx}2500 genes are upregulated >3-fold, of which {approx}900 are also expressed in Jurkat cells. Concomitantly, {approx}1500 genes are downregulated or repressed, of which {approx}500 are also downregulated in Jurkat cells. Gene expression changes persist for over 30 passages ({approx}80 population doublings) in culture. Target cell-type specificity of these changes is shown by the lack of activation or repression of Jurkat-specific genes by extracts of 293T cells or carcinoma cells. Quantitative RT-PCR analysis confirms the long-term transcriptional activation of genes involved in key T cell functions. Additionally, growth of cells in suspended aggregates, expression of CD3 and CD28 T cell surface markers, and interleukin-2 secretion by 293T cells treated with extract of adult peripheral blood T cells illustrate a functional nuclear reprogramming. Therefore, target cell-type-specific and heritable changesmore »
- Authors:
-
- Institute of Basic Medical Sciences, Department of Biochemistry, University of Oslo, PO Box 1112 Blindern, 0317 Oslo (Norway)
- Institute of Basic Medical Sciences, Department of Nutrition Research, University of Oslo, PO Box 1112 Blindern, 0317 Oslo (Norway)
- Publication Date:
- OSTI Identifier:
- 20717649
- Resource Type:
- Journal Article
- Journal Name:
- Experimental Cell Research
- Additional Journal Information:
- Journal Volume: 309; Journal Issue: 1; Other Information: DOI: 10.1016/j.yexcr.2005.06.001; PII: S0014-4827(05)00270-3; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; BLOOD; CARCINOMAS; GENES; IN VITRO; KIDNEYS; POLYMERASE CHAIN REACTION; SECRETION; TRANSCRIPTION
Citation Formats
Hakelien, Anne-Mari, Gaustad, Kristine G, Taranger, Christel K, Skalhegg, Bjorn S, Kuentziger, Thomas, and Collas, Philippe. Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression. United States: N. p., 2005.
Web. doi:10.1016/j.yexcr.2005.06.001.
Hakelien, Anne-Mari, Gaustad, Kristine G, Taranger, Christel K, Skalhegg, Bjorn S, Kuentziger, Thomas, & Collas, Philippe. Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression. United States. https://doi.org/10.1016/j.yexcr.2005.06.001
Hakelien, Anne-Mari, Gaustad, Kristine G, Taranger, Christel K, Skalhegg, Bjorn S, Kuentziger, Thomas, and Collas, Philippe. 2005.
"Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression". United States. https://doi.org/10.1016/j.yexcr.2005.06.001.
@article{osti_20717649,
title = {Long-term in vitro, cell-type-specific genome-wide reprogramming of gene expression},
author = {Hakelien, Anne-Mari and Gaustad, Kristine G and Taranger, Christel K and Skalhegg, Bjorn S and Kuentziger, Thomas and Collas, Philippe},
abstractNote = {We demonstrate a cell extract-based, genome-wide and heritable reprogramming of gene expression in vitro. Kidney epithelial 293T cells have previously been shown to take on T cell properties following a brief treatment with an extract of Jurkat T cells. We show here that 293T cells exposed for 1 h to a Jurkat cell extract undergo genome-wide, target cell-type-specific and long-lasting transcriptional changes. Microarray analyses indicate that on any given week after extract treatment, {approx}2500 genes are upregulated >3-fold, of which {approx}900 are also expressed in Jurkat cells. Concomitantly, {approx}1500 genes are downregulated or repressed, of which {approx}500 are also downregulated in Jurkat cells. Gene expression changes persist for over 30 passages ({approx}80 population doublings) in culture. Target cell-type specificity of these changes is shown by the lack of activation or repression of Jurkat-specific genes by extracts of 293T cells or carcinoma cells. Quantitative RT-PCR analysis confirms the long-term transcriptional activation of genes involved in key T cell functions. Additionally, growth of cells in suspended aggregates, expression of CD3 and CD28 T cell surface markers, and interleukin-2 secretion by 293T cells treated with extract of adult peripheral blood T cells illustrate a functional nuclear reprogramming. Therefore, target cell-type-specific and heritable changes in gene expression, and alterations in cell function, can be promoted by extracts derived from transformed cells as well as from adult primary cells.},
doi = {10.1016/j.yexcr.2005.06.001},
url = {https://www.osti.gov/biblio/20717649},
journal = {Experimental Cell Research},
issn = {0014-4827},
number = 1,
volume = 309,
place = {United States},
year = {Sat Sep 10 00:00:00 EDT 2005},
month = {Sat Sep 10 00:00:00 EDT 2005}
}