Nuclear actin is partially associated with Cajal bodies in human cells in culture and relocates to the nuclear periphery after infection of cells by adenovirus 5
- School of Biochemistry and Molecular Biology, University of Leeds, Leeds, LS2 9JT (United Kingdom)
- CRUK Clinical Centre at Leeds, St. James' University Hospital, Leeds, LS9 7TF (United Kingdom)
Cajal bodies are intra-nuclear structures enriched in proteins involved in transcription and mRNA processing. In this study, immunofluorescence microscopy experiments using a highly specific antibody to actin revealed nuclear actin spots that colocalized in part with p80 coilin-positive Cajal bodies. Actin remained associated with Cajal bodies in cells extracted to reveal the nuclear matrix. Adenovirus infection, which is known to disassemble Cajal bodies, resulted in loss of actin from these structures late in infection. In infected cells, nuclear actin was observed to relocate to structures at the periphery of the nucleus, inside the nuclear envelope. Based on these findings, it is suggested that actin may play an important role in the organization or function of the Cajal body.
- OSTI ID:
- 20717531
- Journal Information:
- Experimental Cell Research, Vol. 303, Issue 2; Other Information: DOI: 10.1016/j.yexcr.2004.06.034; PII: S0014-4827(04)00511-7; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
Similar Records
Human UBL5 protein interacts with coilin and meets the Cajal bodies
Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies