Role of fascin in the proliferation and invasiveness of esophageal carcinoma cells

Journal Article · Fri Nov 11 00:00:00 EST 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.09.055· OSTI ID:20713437

Xie, J J ^[1]; Xu, L Y ^[2]; Zhang, H H ^[1]; Cai, W J ^[3]; Mai, R Q ^[1]; Xie, Y M ^[4]; Yang, Z M ^[1]; Niu, Y D ^[1]; Shen, Z Y ^[3]; Li, E M ^[1]

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1. Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041 (China)
2. College of Life Science and Biological Engineering, Beijing University of Technology, Beijing 100022 (China)
3. Department of Pathology, Medical College of Shantou University, Shantou 515041 (China)
4. Department of Experimental Animal Center, Medical College of Shantou University, Shantou 515041 (China)

Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility in various transformed cells. The overexpression of fascin in esophageal squamous cell carcinoma (ESCC) has been described only recently, but the roles and mechanism still remained unclear. Here, by using RNA interference (RNAi), we have stably silenced the expression of the fascin in EC109 cells, an ESCC cell line. Down-regulation of fascin resulted in a suppression of cell proliferation and as well as a decrease in cell invasiveness. Furthermore, we revealed that fascin might have functions in regulating tumor growth in vivo. The effect of fascin on cell invasiveness correlated with the activation of matrix metalloproteases such as MMP-2 and MMP-9. We examined that fascin down-expression also led to a decrease of c-erbB-2 and {beta}-catenin at the protein level. These results suggested that fascin might play crucial roles in regulating neoplasm progression of ESCC.

• OSTI ID: 20713437
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 337, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.09.055; PII: S0006-291X(05)02056-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ACTIN
CARCINOMAS
CELL PROLIFERATION
GENE REGULATION
GROWTH
IN VIVO
INHIBITION
RNA