Opposing actions of TGF{beta} and MAP kinase signaling in undifferentiated hen granulosa cells

Journal Article · Fri Oct 21 00:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.08.107· OSTI ID:20713383

Woods, Dori C ^[1]; Haugen, Morgan J ^[1]; Johnson, A L ^[1]

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1. Department of Biological Sciences, University of Notre Dame, P.O. Box 369, Notre Dame, IN 46556 (United States)

The present studies were conducted to establish interactions between transforming growth factor (TGF)-{beta} and the epidermal growth factor (EGF) family members, TGF{alpha} and betacellulin (BTC), relative to proliferation and differentiation of granulosa cells in hen ovarian follicles. Results presented demonstrate expression of TGF{beta} isoforms, plus TGF{alpha}, BTC, and ErbB receptors in prehierarchal follicles, thus establishing the potential for autocrine/paracrine signaling and cross-talk within granulosa cells at the onset of differentiation. Treatment with TGF{alpha} or BTC increases levels of TGF{beta}1 mRNA in undifferentiated granulosa cells, while the selective inhibitor of mitogen activated protein kinase signaling, U0126, reverses these effects. Moreover, TGF{beta}1 attenuates c-myc mRNA expression and granulosa cell proliferation, while TGF{alpha} blocks both these inhibitory effects. Collectively, these data provide evidence that EGF family ligands regulate both the expression and biological actions of TGF{beta}1 in hen granulosa cells, and indicate that the timely interaction of these opposing factors is an important modulator of both granulosa cell proliferation and differentiation.

• OSTI ID: 20713383
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 336, Issue 2; Other Information: DOI: 10.1016/j.bbrc.2005.08.107; PII: S0006-291X(05)01800-0; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
CHICKENS
FSH
GROWTH FACTORS
LIGANDS
RECEPTORS