Expression of the multifunctional Y-box protein, YB-1, in myofibroblasts of the infarcted rat heart
- Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163 (United States)
- Division of Cardiovascular Diseases, University of Tennessee Health Science Center, Memphis, TN 38163 (United States)
Intracellular signaling mechanisms regulating the turnover of {alpha}-SMA-positive myofibroblasts (myoFbs) at the site of myocardial infarction (MI) are poorly understood. Y-Box (YB)-1, a multifunctional protein, may be involved in regulation of proliferation, migration and apoptosis of myoFbs. Our objective was to study the expression of YB-1 in the infarcted rat heart and its localization in myoFbs. On days 3-28 following MI, we monitored YB-1 expression and its colocalization with {alpha}-SMA, and proliferation markers PCNA and Ki-67 in infarcted tissue by Western blot, immunohistochemistry, and immunofluorescent double-labeling. YB-1 is barely detectable in normal myocardium. At the infarct site, however, YB-1 is markedly elevated from day 3 post-MI concomitant with the induction of cell proliferation. MyoFbs are the major source of YB-1 and retain it up to day 28 post-MI. We suggest early expression of YB-1 promotes proliferation and migration of myoFbs, whereas prolonged expression may be responsible for scar formation.
- OSTI ID:
- 20710918
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 334, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.06.082; PII: S0006-291X(05)01292-1; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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