Photodynamic effects of a novel pterin derivative on a pancreatic cancer cell line
- Department of Hematology and Oncology, Kyoto University Hospital, Kyoto 606-8507 (Japan)
- Department of Anesthesia, Kyoto University Hospital, Kyoto 606-8507 (Japan)
- Wakasa Wan Energy Research Center, Tsuruga 914-0129 (Japan)
- Institute of Advanced Energy, Kyoto University, Uji 611-0011 (Japan)
- School of Health Science Faculty of Medicine, Kyoto University, Kyoto 606-8507 (Japan)
6-Formylpterin (6FP) has the potential to produce singlet oxygen ({sup 1}O{sub 2}) under UV-A radiation. In order to apply this potential to anti-cancer photodynamic therapy (PDT), we prepared a novel variant of 6FP, 2-(N,N-dimethylaminomethyleneamino)-6-formyl-3-pivaloylpteridine-4-one (6FP-tBu-DMF), and examined its photodynamic effects on a pancreatic cancer cell line, Panc-1 cells. The study using laser scanning confocal microscopy showed that the drug uptake, the {sup 1}O{sub 2} generation, and cell death were observed in the 6FP-tBu-DMF-treated cells, while these phenomena were not observed in the 6FP-treated cells. The MTT assay also showed the decrease in cell viability only in the 6FP-tBu-DMF-treated cells. Since 6FP and 6FP-tBu-DMF generate {sup 1}O{sub 2} to the same extent under UV-A radiation in aqueous solutions, these results indicated that the differences in the photodynamic effects between 6FP and 6FP-tBu-DMF were entirely attributed to the differences in the cell permeability between them. The development of cell permeable pterin derivatives has the potential for application in PDT.
- OSTI ID:
- 20710879
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 333, Issue 3; Other Information: DOI: 10.1016/j.bbrc.2005.05.185; PII: S0006-291X(05)01112-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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