Neural differentiation of adipose-derived stem cells isolated from GFP transgenic mice
- Department of Pharmacology, Nippon Medical School, Tokyo (Japan)
- Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo (Japan)
- Department of Pediatrics, Nippon Medical School, Tokyo (Japan)
Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have recently reported that adipose-derived stromal cells (ASCs) could differentiate into mesenchymal lineages in vitro. In this study, we performed neural induction using ASCs from GFP transgenic mice and were able to induce these ASCs into neuronal and glial cell lineages. Most of the neurally induced cells showed bipolar or multipolar appearance morphologically and expressed neuronal markers. Electron microscopy revealed their neuronal morphology. Some cells also showed glial phenotypes, as shown immunocytochemically. The present study clearly shows that ASCs derived from GFP transgenic mice differentiate into neural lineages in vitro, suggesting that these cells might provide an ideal source for further neural stem cell research with possible therapeutic application for neurological disorders.
- OSTI ID:
- 20710860
- Journal Information:
- Biochemical and Biophysical Research Communications, Vol. 333, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.05.096; PII: S0006-291X(05)01078-8; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
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