Spore-displayed streptavidin: A live diagnostic tool in biotechnology

Journal Article · Fri May 27 00:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.03.144· OSTI ID:20709207

Kim, June-Hyung ^[1]; Lee, Chang-Soo ^[2]; Kim, Byung-Gee ^[1]

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1. Institute of Molecular Biology and Genetics, School of Chemical Engineering, Seoul National University (Korea, Republic of)
2. Department of Chemical Engineering, Chungnam National University, Yuseong-Gu, Daejeon 305-764 (Korea, Republic of)

Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-streptavidin antibody was used for the verification of surface localization of expressed CotG-streptavidin fusion protein. FACS and dot-blot were used for the verification of biological activity of displayed streptavidin with FITC-labeled biotin.

• OSTI ID: 20709207
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 331, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.03.144; PII: S0006-291X(05)00611-X; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBODIES
BACILLUS SUBTILIS
BIOTECHNOLOGY
BIOTIN
PROTEINS
SPORES
VERIFICATION