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Title: Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids

Abstract

Synchrotron Radiation micro X-Ray Fluorescence (SR {mu}-XRF) was applied for the first time to map the trace element content on Multicellular Tumor Spheroids (MTS), i.e. human cell clusters used as an in vitro model for testing micrometastases responses to antitumoral drugs. In particular, immunotoxin molecules composed of a carrier protein (Transferrin) bound to a powerful cytotoxin (Ricin A), were here considered as representatives of a class of therapheutic macromolecules used in cancer theraphy. Spheroids included in polyacrylamide gel and placed inside quartz capillaries were studied at the ESRF ID22 beamline using a 15 keV monochromatic photon microbeam. Elemental maps (of Fe, Cu, Zn and Pb) on four groups of spheroids grown under different conditions were studied: untreated, treated only with the carrier molecule or with the toxin alone, and with the complete immunotoxin molecule (carrier+toxin). The results indicate that the distribution of Zn and, to some extent, Cu in the spheroid cells is homogeneous and independent of the treatment type. Total Reflection X-Ray Fluorescence (TR-XRF) was also applied to quantify the average trace element content in the spheroids. Future developments of the technique are finally outlined on the basis of these preliminary results.

Authors:
 [1];  [1]; ; ;  [2];  [3]
  1. Istituto Nazionale di Fisica Nucleare- Laboratori di Frascati, via E. Fermi 40, I-00044 Frascati (Italy)
  2. Universita degli Studi di Verona-Dip. Patologia, Sez. Immunologia, P.le A.L. Scuro, I-37134 Verona (Italy)
  3. Universita degli Studi di Verona-Dipartimento di Informatica, Strada LeGrazie 15, I-37134 Verona (Italy)
Publication Date:
OSTI Identifier:
20636491
Resource Type:
Journal Article
Journal Name:
AIP Conference Proceedings
Additional Journal Information:
Journal Volume: 652; Journal Issue: 1; Conference: 19. International conference on X-ray and inner-shell processes, Rome (Italy), 24-28 Jun 2002; Other Information: DOI: 10.1063/1.1536413; (c) 2003 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-243X
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; 60 APPLIED LIFE SCIENCES; ANIMAL CELLS; CAPILLARIES; EUROPEAN SYNCHROTRON RADIATION FACILITY; FLUORESCENCE; GELS; IN VITRO; KEV RANGE; METALS; MOLECULES; MONOCHROMATIC RADIATION; NEOPLASMS; PHOTONS; QUARTZ; RADIOTHERAPY; REFLECTION; SPHEROIDS; SYNCHROTRON RADIATION; TESTING; TOXINS; TRANSFERRIN; X RADIATION; X-RAY FLUORESCENCE ANALYSIS

Citation Formats

Burattini, E, Universita degli Studi di Verona -Dipartimento di Informatica, Strada LeGrazie 15, I-37134 Verona, Cinque, G, Bellisola, G, Fracasso, G, Colombatti, M, and Monti, F. Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids. United States: N. p., 2003. Web. doi:10.1063/1.1536413.
Burattini, E, Universita degli Studi di Verona -Dipartimento di Informatica, Strada LeGrazie 15, I-37134 Verona, Cinque, G, Bellisola, G, Fracasso, G, Colombatti, M, & Monti, F. Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids. United States. https://doi.org/10.1063/1.1536413
Burattini, E, Universita degli Studi di Verona -Dipartimento di Informatica, Strada LeGrazie 15, I-37134 Verona, Cinque, G, Bellisola, G, Fracasso, G, Colombatti, M, and Monti, F. 2003. "Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids". United States. https://doi.org/10.1063/1.1536413.
@article{osti_20636491,
title = {Synchrotron Radiation {mu}-X Ray Fluorescence on Multicellular Tumor Spheroids},
author = {Burattini, E and Universita degli Studi di Verona -Dipartimento di Informatica, Strada LeGrazie 15, I-37134 Verona and Cinque, G and Bellisola, G and Fracasso, G and Colombatti, M and Monti, F},
abstractNote = {Synchrotron Radiation micro X-Ray Fluorescence (SR {mu}-XRF) was applied for the first time to map the trace element content on Multicellular Tumor Spheroids (MTS), i.e. human cell clusters used as an in vitro model for testing micrometastases responses to antitumoral drugs. In particular, immunotoxin molecules composed of a carrier protein (Transferrin) bound to a powerful cytotoxin (Ricin A), were here considered as representatives of a class of therapheutic macromolecules used in cancer theraphy. Spheroids included in polyacrylamide gel and placed inside quartz capillaries were studied at the ESRF ID22 beamline using a 15 keV monochromatic photon microbeam. Elemental maps (of Fe, Cu, Zn and Pb) on four groups of spheroids grown under different conditions were studied: untreated, treated only with the carrier molecule or with the toxin alone, and with the complete immunotoxin molecule (carrier+toxin). The results indicate that the distribution of Zn and, to some extent, Cu in the spheroid cells is homogeneous and independent of the treatment type. Total Reflection X-Ray Fluorescence (TR-XRF) was also applied to quantify the average trace element content in the spheroids. Future developments of the technique are finally outlined on the basis of these preliminary results.},
doi = {10.1063/1.1536413},
url = {https://www.osti.gov/biblio/20636491}, journal = {AIP Conference Proceedings},
issn = {0094-243X},
number = 1,
volume = 652,
place = {United States},
year = {Fri Jan 24 00:00:00 EST 2003},
month = {Fri Jan 24 00:00:00 EST 2003}
}