Staphylococcal surface display of metal-binding polyhistidyl peptides
Recombinant Staphylococcus xylosus and Staphylococcus carnosus strains were generated with surface-exposed chimeric proteins containing polyhistidyl peptides designed for binding to divalent metal ions. Surface accessibility of the chimeric surface proteins was demonstrated and the chimeric surface proteins were found to be functional in terms of metal binding, since the recombinant staphylococcal cells were shown to have gained Ni{sup 2+}- and Cd{sup 2+}-binding capacity, suggesting that such bacteria could find use in bioremediation of heavy metals. This is, to their knowledge, the first time that recombinant, surface-exposed metal-binding peptides have been expressed on gram-positive bacteria. Potential environmental or biosensor applications for such recombinant staphylococci as biosorbents are discussed.
- Research Organization:
- Kungliga Tekniska Hoegskolan, Stockholm (SE)
- OSTI ID:
- 20026668
- Journal Information:
- Applied and Environmental Microbiology, Vol. 66, Issue 3; Other Information: PBD: Mar 2000; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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