Detection of nucleic acid sequences by invader-directed cleavage
Abstract
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5{prime} nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.
- Inventors:
- Publication Date:
- Research Org.:
- US Department of Energy, Washington, DC (US)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 20013254
- Application Number:
- Int. Cl. C12Q 1/68; C12P 19/34; C07H 21/02; US 6,001,567/A/; 8-682,853; TRN: IM200010%%122
- Assignee:
- PTO; EDB-00:006676
- DOE Contract Number:
- FG02-94ER81891
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 14 Dec 1999
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; NUCLEIC ACIDS; DNA SEQUENCING; DESIGN; DNA SEQUENCERS; CLEAVAGE; NUCLEASES; ENZYME ACTIVITY
Citation Formats
Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, and Prudent, J R. Detection of nucleic acid sequences by invader-directed cleavage. United States: N. p., 1999.
Web.
Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, & Prudent, J R. Detection of nucleic acid sequences by invader-directed cleavage. United States.
Brow, M A.D., Hall, J S.G., Lyamichev, V, Olive, D M, and Prudent, J R. 1999.
"Detection of nucleic acid sequences by invader-directed cleavage". United States.
@article{osti_20013254,
title = {Detection of nucleic acid sequences by invader-directed cleavage},
author = {Brow, M A.D. and Hall, J S.G. and Lyamichev, V and Olive, D M and Prudent, J R},
abstractNote = {The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5{prime} nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.},
doi = {},
url = {https://www.osti.gov/biblio/20013254},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 14 00:00:00 EST 1999},
month = {Tue Dec 14 00:00:00 EST 1999}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.