Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology
Patent
·
OSTI ID:20013225
An enhanced strain of Pseudomonas cellulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain {number{underscore}sign}142 ATCC 55702.
- Research Organization:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-96OR22464
- Assignee:
- ORNL; EDB-00:006648
- Application Number:
- Int. Cl. C12P 19/14; C12N 9/42; US 5,958,740/A/; 8-934,883; TRN: IM200010%%93
- OSTI ID:
- 20013225
- Resource Relation:
- Other Information: PBD: 28 Sep 1999
- Country of Publication:
- United States
- Language:
- English
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