Microdialysis unit for molecular weight separation
The present invention relates generally to an apparatus and method for separating high molecular weight molecules from low molecular weight molecules. More specifically, the invention relates to the use of microdialysis for removal of the salt (low molecular weight molecules) from a nucleotide sample (high molecular weight molecules) for ESI-MS analysis. The dialysis or separation performance of the present invention is improved by (1) increasing dialysis temperature thereby increasing desalting efficiency and improving spectrum quality; (2) adding piperidine and imidazole to the dialysis buffer solution and reducing charge states and further increasing detection sensitivity for DNA; (3) using low concentrations of dialysis buffer and shifting the DNA negative ions to higher charge states, producing a nearly 10-fold increase in detection sensitivity and a slightly decreased desalting efficiency, or (4) any combination of (1), (2), and (3).
- Research Organization:
- PNNL (Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- PNNL; EDB-00:006644
- Application Number:
- Int. Cl. B01D 61/28; B01D 63/02; US 5,954,959/A/; 8-855,727; TRN: IM200010%%89
- OSTI ID:
- 20013221
- Resource Relation:
- Other Information: PBD: 21 Sep 1999
- Country of Publication:
- United States
- Language:
- English
Similar Records
Apparatus for molecular weight separation
On-line microdialysis sample cleanup for electrospray ionization mass spectrometry of nucleic acid samples