Steady-state and transient-state analysis of growth and metabolite production in a Saccharomyces cerevisiae strain with reduced pyruvate-decarboxylase activity
Pyruvate decarboxylase is a key enzyme in the production of low-molecular-weight byproducts (ethanol, acetate) in biomass-directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2 gene, which encodes a positive regulator of pyruvate-decarboxylase synthesis, was inactivated in the prototrophic strain S. cerevisiae CEN.PK113-7D. This caused a 3--4-fold reduction of pyruvate-decarboxylase activity in glucose-limited, aerobic chemostat cultures grown at a dilution rate of 0.10 h{sup {minus}1}. Upon exposure of such cultures to a 50 mM glucose pulse, ethanol and acetate were the major byproducts formed by the wild type. In the pdc2{Delta} strain, formation of ethanol and acetate was reduced by 60--70%. In contrast to the wild type, the pdc2{Delta} strain produced substantial amounts of pyruvate after a glucose pulse. Nevertheless, its overall byproduct formation was ca. 50% lower. The specific rate of glucose consumption after a glucose pulse to pdc2{Delta} cultures was about 40% lower than in wild-type cultures.
- Research Organization:
- Delft Univ. of Technology (NL)
- OSTI ID:
- 20006611
- Journal Information:
- Biotechnology and Bioengineering, Vol. 66, Issue 1; Other Information: PBD: 1999; ISSN 0006-3592
- Country of Publication:
- United States
- Language:
- English
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