Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?

Gerritsen, A A.M.; Hoeven, N van der

Title: Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?

Full Record
Other Related Research

Abstract

Long term consequences associated with exposure to endocrine disrupting chemicals in the environment have been found in mammals, birds, fish, turtles and gastropods. Despite their important role, however, hardly any attention has been paid to the long term effects of such chemicals on crustaceans. Experiments originally carried out to quantify the ability of Daphnia magna to recover from short term exposure to para-tert-pentylphenol, revealed the endocrine disrupting properties of the test compound. During one of the experiments animals were divided into six (8 hour) age groups between 0 and 48 hours and exposed to 6 mg of para-tert-pentylphenol 1{sup {minus}1} for a period of 8 hours. Within the age groups of 16 to 24 and of 24 to 32 hours old 51 and 70% of the females respectively lag-fed behind in growth and showed reduced fertility. In addition to this, about 37 and 16% of these females showed malformations suggesting defeminization. The males showed no delay in growth and had no malformations. Furthermore, in terms of survival the males were shown to be less sensitive towards the test compound than the females. The type of effects in females and the sharp distinction between the effects on males and females aremore »« less

Authors:: Gerritsen, A A.M.; Hoeven, N van der

Publication Date:: Sun Dec 31 00:00:00 EST 1995

OSTI Identifier:: 197550

Report Number(s):: CONF-9511137-
ISBN 1-880611-03-1; TRN: IM9612%%324

Resource Type:: Conference

Resource Relation:: Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.

Country of Publication:: United States

Language:: English

Subject:: 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; PHENOLS; BIOASSAY; DAPHNIA; SENSITIVITY; ESTROGENS; RECEPTORS; ENDOCRINE GLANDS; SEX DEPENDENCE; BIOCHEMICAL REACTION KINETICS; METABOLISM

Citation Formats


                    Gerritsen, A A.M., and Hoeven, N van der. Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?.  United States: N. p., 1995. 
        Web.

Copy to clipboard


                    Gerritsen, A A.M., & Hoeven, N van der. Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?.  United States.

Copy to clipboard


                    Gerritsen, A A.M., and Hoeven, N van der. 1995.  
        "Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?".  United States.

Copy to clipboard


                    
@article{osti_197550,

  title        = {Defeminization in Daphnia magna: A screening test for endocrine-disruptors in the environment?},

  author       = {Gerritsen, A A.M. and Hoeven, N van der},

  abstractNote = {Long term consequences associated with exposure to endocrine disrupting chemicals in the environment have been found in mammals, birds, fish, turtles and gastropods. Despite their important role, however, hardly any attention has been paid to the long term effects of such chemicals on crustaceans. Experiments originally carried out to quantify the ability of Daphnia magna to recover from short term exposure to para-tert-pentylphenol, revealed the endocrine disrupting properties of the test compound. During one of the experiments animals were divided into six (8 hour) age groups between 0 and 48 hours and exposed to 6 mg of para-tert-pentylphenol 1{sup {minus}1} for a period of 8 hours. Within the age groups of 16 to 24 and of 24 to 32 hours old 51 and 70% of the females respectively lag-fed behind in growth and showed reduced fertility. In addition to this, about 37 and 16% of these females showed malformations suggesting defeminization. The males showed no delay in growth and had no malformations. Furthermore, in terms of survival the males were shown to be less sensitive towards the test compound than the females. The type of effects in females and the sharp distinction between the effects on males and females are indicative for the estrogenicity of para-tert-pentylphenol. The estrogenic effects of a number alkylphenols, including para-tert-pentylphenol, were demonstrated in rats and confirmed in tests with the human breast cell MCF7. To the best of knowledge estrogenicity has not been recorded before for any chemical for any crustacean. The observation of estrogenic effects in D. magna opens up the way to a standardized test for screening chemicals with potentially endocrine disrupting properties. Such a test may be a valuable tool in environmental risk assessment and conservation of environmental and human health.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/197550},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 00:00:00 EST 1995},

  month        = {Sun Dec 31 00:00:00 EST 1995}

}

Copy to clipboard

Conference:

Other availability

Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Ecological risk assessment of substances with suspected estrogenic activity using standard laboratory fish tests

Conference Gimeno, S; Bowmer, C

The assessment of risks to the aquatic environment in the European Union is generally based on a comparison of Predicted Environmental Concentrations (PEC) with Predicted No Effect Concentrations (PNEC) for surrogate, or representative, organisms of the receiving waters. Such risk assessments are required for new and priority existing chemicals, pesticides, and, in the near future, biocides; they are dependent on robust in vivo test data. Current strategies for ecological risk assessment were not designed to assess the risk of endocrine disrupters. The selection of suitable fish species and practical in vivo end points for determining endocrine disruption in fish aremore »« less
Physiological and biochemical perturbations in Daphnia magna following exposure to the model environmental estrogen diethylstilbestrol

Journal Article Baldwin, W; Milam, D; LeBlanc, G - Environmental Toxicology and Chemistry

The estrogenic properties of many environmental contaminants, such as DDE and PCBs, have been associated with reproductive failure in a variety of vertebrate species. While estrogens have been measured in many invertebrate species, the function of this hormone in invertebrates is controversial. The objective of the present study was to identify possible physiological and biochemical target sites for the estrogenic effects of some xenobiotics on the freshwater crustacean Daphnia magna using the model environmental estrogen diethylstilbestrol (DES). Chronic exposure of daphnids to 0.50 mg/L DES reduced molting frequency among first-generation juveniles and decreased fecundity of second-generation daphnids. Adult first-generation daphnidsmore »« less
https://doi.org/10.1897/1552-8618(1995)14[945:PABPID]2.0.CO;2
Development of quantitative real-time PCR assays for fathead minnow (Pimephales promelas) gonadotropin ß subunit mRNAs to support endocrine disruptor research.

Journal Article Villeneuve, Daniel; Miracle, Ann; Jensen, Kathleen; ... - Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 145(2):171-183

Fathead minnows (Pimephales promelas) are one of the most widely-used small fish models for regulatory ecotoxicology testing and research related to endocrine disrupting chemicals (EDCs). In this study, we isolated and sequenced cDNAs for fathead minnow follicle-stimulating hormone-like and luteinizing hormone-like β (FSHβ and LHβ) and glycoprotein α (GPα) subunits. Quantitative real-time PCR assays for measuring gonadotropin (GtH) β subunit transcripts were developed and used to examine “baseline” transcript levels over a range of age classes and reproductive states encompassed in EDC testing. In females, FSHβ and LHβ transcripts were greater in 4-5 month old than in younger fish andmore »« less
https://doi.org/10.1016/j.cbpc.2006.11.003
Use of medaka (Oryzias latipes) as a model teleost to identify reproductive endpoints indicative of exposure to endocrine disrupting chemicals

Conference Papoulias, D; Tillitt, D; Jones, S; ...

The purpose of this research is to identify effects of endocrine disrupting chemicals on the reproductive system of fish at various stages of sexual differentiation and development. The model teleost used was the d-rR strain of medaka (Oryzias latipes) which has a heritable sex-linked color gene. In medaka, sex differences are first detected at the cellular level at hatch. In males, the mitotic activity of the primordial germ cells (PGC) is arrested while the PGCs of the females continue to divide. The germ cells of the gonochoristic medaka have been demonstrated to be totipotent with respective to gonia production. Becausemore »« less
Environmental endocrine disruptors: A proposed classification scheme

Conference Fur, P.L.; Roberts, J

A number of chemicals known to act on animal systems through the endocrine system have been termed environmental endocrine disruptors. This group includes some of the PCBs and TCDDs, as well as lead, mercury and a large number of pesticides. The common feature is that the chemicals interact with endogenous endocrine systems at the cellular and/or molecular level to alter normal processes that are controlled or regulated by hormones. Although the existence of artificial or environmental estrogens (e.g. chlordecone and DES) has been known for some time, recent data indicate that this phenomenon is widespread. Indeed, anti-androgens have been heldmore »« less

Similar Records