Harmonization of standard toxicity test methods used in North America
- NBS, Columbia, MO (United States)
- Environmental Protection Agency, Duluth, MN (United States); and others
Over the past two years, Environment Canada (EC) and the US Environmental Protection Agency (EPA) have developed standard methods for conducting toxicity and bioaccumulation tests with freshwater, estuarine, and marine sediments. Existing ASTM methods were used as a basis to harmonize these methods for conducting testing with either field-collected or laboratory-spiked sediments. For freshwater toxicity tests, methods are described by EC and EPA for the amphipod Hyalella azteca and the midges Chironomus tentans and C. riparius. Endpoints include 10- to 14-d survival of growth. Methods are also described by EPA for conducting 28-d bioaccumulation tests with the oligochaete Lumbriculus variegatus. For estuarine and marine toxicity tests, methods are described for several amphipods (i.e., Rhepoxynius abronius, Ampelisca abdita, Eohaustorius estuarius, Leptocheirus plumulosus). Endpoints include 10-d survival and reburial. EC is also developing methods for conducting toxicity tests with Atlantic, Pacific, and Arctic Canadian species of polychaetes. Methods are described by EPA for conducting 28-d bioaccumulation tests with a variety of mollusks (i.e., Macoma spp.) and polychaetes (i.e., Nereis spp.). Slight inconsistencies in methods between freshwater and estuarine/marine testing or between EC and EPA testing include: (1) static vs. flow-through conditions, (2) sieving of sediment, (3) types and quantity of food, (4) age of test organisms, or (4) duration of the test and required endpoints. Additional research is in progress to: (1) develop chronic toxicity tests with amphipods and midges measuring survival, growth, or reproduction, (2) develop whole-sediment toxicity identification evaluation (TIE) procedures, (3) refine sediment spiking procedures, and (4) field-validate laboratory tests.
- OSTI ID:
- 191300
- Report Number(s):
- CONF-9511137-; ISBN 1-880611-03-1; TRN: IM9610%%301
- Resource Relation:
- Conference: 2. Society of Environmental Toxicology and Chemistry (SETAC) world conference, Vancouver (Canada), 5-9 Nov 1995; Other Information: PBD: 1995; Related Information: Is Part Of Second SETAC world congress (16. annual meeting): Abstract book. Global environmental protection: Science, politics, and common sense; PB: 378 p.
- Country of Publication:
- United States
- Language:
- English
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